Redox metabolism plays an essential role in the central metabolic network of all living cells, connecting, but at the same time separating, catabolic and anabolic pathways. Redox metabolism is inherently linked to the excretion of overflow metabolites. Overflow metabolism allows for higher substrate uptake rates, potentially outcompeting other microorganisms for the same substrate. Within dynamically changing environments, overflow metabolism can act as storage mechanism, as is shown in many recently described processes. However, for complete understanding of these mechanisms, the intracellular state of the metabolism must be elucidated. In recent years, progress has been made in the field of metabolomics to improve the accuracy and precision of measurements of intracellular and intercompartmental metabolites. This article highlights several of these recent advances, with focus on redox cofactor measurements, both fluorescence and mass spectrometry based.

中文翻译：

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从化学计量学到动力学的氧化还原代谢动力学。

氧化还原代谢在所有活细胞的中央代谢网络中起着至关重要的作用，它们相互连接，但同时又分解代谢途径和合成代谢途径。氧化还原代谢固有地与溢出代谢物的排泄有关。溢出新陈代谢允许更高的底物吸收率，可能在同一底物上超过其他微生物。在动态变化的环境中，溢流新陈代谢可以充当存储机制，如许多最近描述的过程所示。但是，为了完全理解这些机制，必须阐明新陈代谢的细胞内状态。近年来，在代谢组学领域已取得进展，以提高细胞内和室间代谢物的测量准确性和精确度。