Tiopronin (MPG) is a thiol antioxidant drug that has been explored as a treatment for various oxidative stress-related disorders. However, many of its antioxidant capabilities remain untested in well-validated cell models. To more thoroughly understand the action of this promising pharmaceutical compound against acute oxidative challenge, A549 human lung carcinoma cells were exposed to tert-butyl hydroperoxide (tBHP) and treated with MPG. Analyses of cell viability, intracellular glutathione (GSH) levels, and the prevalence of reactive oxygen species (ROS) and mitochondrial superoxide were used to examine the effects of MPG on tBHP-challenged cells. MPG treatment suppressed intracellular ROS and mitochondrial superoxide and prevented tBHP-induced GSH depletion and apoptosis. These results indicate that MPG is effective at preserving redox homeostasis against acute oxidative insult in A549 cells if present at sufficient concentrations during exposure to oxidants such as tBHP. The effects of treatment gleaned from this study can inform experimental design for future in vivo work on the therapeutic potential of MPG.

硫普罗宁（MPG）是一种硫醇抗氧化剂，已被研究用于治疗各种与氧化应激相关的疾病。但是，其许多抗氧化功能仍未在经过充分验证的细胞模型中进行测试。为了更透彻地了解这种有希望的药物化合物对抗急性氧化挑战的作用，将A549人肺癌细胞暴露于叔丁基过氧化氢（t BHP）中并用MPG处理。细胞活力，细胞内谷胱甘肽（GSH）水平，活性氧（ROS）和线粒体超氧化物的流行率分析用于检测MPG对受t BHP攻击的细胞的影响。MPG处理可抑制细胞内ROS和线粒体超氧化物并预防tBHP诱导的GSH耗竭和凋亡。这些结果表明，如果在暴露于诸如t BHP等氧化剂的过程中以足够的浓度存在，MPG可以有效地保持氧化还原稳态以抵抗A549细胞中的急性氧化损伤。从这项研究中获得的治疗效果可以为未来体内MPG治疗潜力的实验设计提供依据。