Abstract

This study aimed to evaluate the in vitro activity of extracts of two marine sponge species, occurring in the shallows of the Yucatan peninsula coast, on two cancer and one normal mammalian cell lines. Hexane, dichloromethane, ethyl acetate and methanol extracts of Halichondria magniconulosa and Halichondria melanadocia were screened for their cytotoxic activity against hormone-dependent breast cancer (MCF-7) and human cervix cancer (SiHa) cell lines. The ethyl acetate extract of H. magniconulosa exhibited significant cytotoxicity against MCF-7 cells to a CC₅₀ of 0.8 µg/mL, as well as high selectivity (SI = 24.5). On the other hand, SiHa cells were moderately sensitive to the dichloromethane and ethyl acetate extracts of the same species. (CC₅₀ = 34.9 and 31.5 µg/mL, respectively). None of the extracts of H. melanadocia were considered active due their CC₅₀’s were ranged from 59.0 to 94.5 µg/mL.

摘要

本研究旨在评估出现在尤卡坦半岛海岸浅滩的两种海绵物种的提取物对两种癌症和一种正常哺乳动物细胞系的体外活性。筛选了Halichondria magniconulosa和Halichondria melanadocia 的己烷、二氯甲烷、乙酸乙酯和甲醇提取物对激素依赖性乳腺癌 (MCF-7) 和人类宫颈癌 (SiHa) 细胞系的细胞毒活性。H. magniconulosa的乙酸乙酯提取物对 MCF-7 细胞对 CC ₅₀表现出显着的细胞毒性0.8 µg/mL，以及高选择性 (SI = 24.5)。另一方面，SiHa 细胞对同一物种的二氯甲烷和乙酸乙酯提取物中度敏感。（CC ₅₀ = 34.9 和 31.5 µg/mL，分别）。由于其 CC ₅₀的范围在 59.0 至 94.5 µg/mL 之间，因此没有任何黑果树提取物被认为具有活性。