Abstract

Ruellia angustiflora (Acanthaceae) is known as flower-of-fire, and its leaves are traditionally employed to promote wound healing. This study was aimed at extracting and characterizing the chemical constituents of the extracts of R. angustiflora obtained by ultrasound-assisted and supercritical fluid extractions, and subsequently investigate their antioxidant potential and cyto-genotoxicity. The extract obtained by ultrasound (UAE-EtOH) was characterized by ultraperformance liquid chromatography-mass spectrometer (UPLC-MS), and the extract obtained via supercritical fluid (SFE-CO₂) by gas chromatograph-mass spectrometer (GC-MS). The antioxidant potential was verified by the antiradical activity against the 1,1-diphenyl-2-picrylhydrazyl radical (DPPH), and the cyto-genotoxicity evaluation to test cell viability and DNA damage was performed in peripheral blood mononuclear cells (PBMC) cultures. The SFE-CO₂ extract presented some fatty acids, triterpenes, tetraterpenes, tocopherols and phytosterols. The UAE-EtOH extract contained phenolic acids and flavonoids, and showed the highest antioxidant capacity. Neither extract was genotoxic or cytotoxic at the tested concentrations.

摘要

Ruellia angustiflora (Acanthaceae) 被称为火之花，其叶子传统上用于促进伤口愈合。本研究旨在提取和表征通过超声辅助和超临界流体提取获得的罗汉果提取物的化学成分，并随后研究它们的抗氧化潜力和细胞遗传毒性。超声（UAE-EtOH）提取物经超高效液相色谱-质谱（UPLC-MS）表征，超临界流体（SFE-CO ₂) 通过气相色谱-质谱仪 (GC-MS)。通过对 1,1-二苯基-2-苦基肼自由基 (DPPH) 的抗自由基活性验证了抗氧化潜力，并在外周血单核细胞 (PBMC) 培养物中进行了细胞遗传毒性评估以测试细胞活力和 DNA 损伤。SFE-CO ₂提取物含有一些脂肪酸、三萜、四萜、生育酚和植物甾醇。阿联酋乙醇提取物含有酚酸和黄酮类化合物，具有最高的抗氧化能力。在测试浓度下，两种提取物均无遗传毒性或细胞毒性。