Tripterygium hypoglaucum hutch (THH) is a plant of the genus tripterygium, which is also known as colquhounia, Gelsemiun elegan, and so on. It is mainly distributed in Yunnan, Guizhou, and Sichuan regions and other places in China. To study the immune mechanism of THH on related inflammatory cytokines in collagen II-induced arthritis (CIA) mice, healthy male C57BL/6 mice were used to model CIA mice. Mice received THH 420 mg/kg/day or the same amount of normal saline (NS) by gavage for 20 days. The thickness of the ankle joint in mice was observed, and the arthritis index was calculated. Related inflammatory cytokines were detected by real-time quantitative polymerase chain reaction and enzyme-linked immunosorbent assay. The results showed that after treatment with THH, the CIA mice had less swelling and destruction of the joints as well as decreased foot size and arthritis index. The mRNA and protein levels of TNF-α, IFN-γ, and IL-17A were lower in the THH-treated group than in the NS group (P < 0.05). In summary, THH has great significance in the treatment of CIA mice, including reduced related inflammatory cytokines expression level in both joint tissue and serum. The mechanism of THH in the treatment of CIA may be through the inhibition of the NF-kB-STAT3-IL-17 pathway, which also requires further experimental investigation.

中文翻译：

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THH 通过减少炎症相关细胞因子来缓解 CIA 炎症。

雷公藤 hypoglaucum hutch (THH) 是雷公藤属植物，也被称为colquhounia、Gelsemiun elegan， 等等。主要分布于云南、贵州、四川等地。为研究 THH 对胶原 II 诱导的关节炎 (CIA) 小鼠相关炎症细胞因子的免疫机制，使用健康雄性 C57BL/6 小鼠对 CIA 小鼠进行建模。小鼠通过强饲法接受 THH 420 mg/kg/天或等量的生理盐水 (NS)，持续 20 天。观察小鼠踝关节厚度，计算关节炎指数。通过实时定量聚合酶链反应和酶联免疫吸附试验检测相关炎性细胞因子。结果表明，在用 THH 治疗后，CIA 小鼠的关节肿胀和破坏较少，足部尺寸和关节炎指数也有所减小。TNF-α、IFN-γ、P  < 0.05）。综上所述，THH 在治疗 CIA 小鼠方面具有重要意义，包括降低关节组织和血清中相关炎性细胞因子的表达水平。THH治疗CIA的机制可能是通过抑制NF-kB-STAT3-IL-17通路，这也需要进一步的实验研究。