In this study, the genetic diversity and the population structure of 77 wild strains and 23 cultivars of Lentinula edodes from Korea were analyzed using 20 genomic SSRs, and their genetic relationship was investigated. The tested strains of L. edodes were divided into three sub-groups consisting of only wild strains, mainly wild strains and several cultivars, and mainly cultivars and several wild strains by distance-based analysis. Using model-based analysis, L. edodes strains were divided into two subpopulations; the first one consisting of only wild strains and the second one with mainly cultivars and several wild strains. Moreover, AMOVA analysis revealed that the genetic variation in the cultivars was higher than that in the wild strains. The expected and observed heterozygosity and values indicating the polymorphic information content of L. edodes cultivars from Korea were also higher than that of the wild strains. Based on these results, we presume that the cultivars in Korea have developed by using numerous strains from other countries. In conclusion, the usage of wild strains for the development of new cultivars could improve the adaptability of L. edodes to biotic and abiotic stress.

中文翻译：

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利用香菇基因组SSR标记分析野生菌株和品种的遗传多样性和种群结构。

本研究利用20个基因组SSRs对韩国77个野生香菇和23个香菇品种的遗传多样性和种群结构进行了分析，并研究了它们的亲缘关系。通过基于距离的分析，被测试的香菇菌株被分成三个亚组，仅由野生菌株组成，主要是野生菌株和几个品种，并且主要是品种和几个野生菌株。使用基于模型的分析，香菇菌株被分为两个亚群。第一个仅由野生菌株组成，第二个主要由栽培品种和几种野生菌株组成。此外，AMOVA分析表明，该品种的遗传变异高于野生菌株。预期和观察到的杂合度和值表明L的多态信息含量。韩国的香菇品种也高于野生品系。根据这些结果，我们推测韩国的栽培品种是通过使用来自其他国家的许多菌株而开发的。总之，将野生菌株用于新品种的开发可以提高香菇对生物和非生物胁迫的适应性。