The long circulation time and targeting drug delivery at tumor sites are still the main challenges of nanodrug delivery systems for antitumor activity. Herein, a cancer cell membrane-coated biomimetic nanodrug delivery system was fabricated. The paclitaxel (PTX)-loaded poly(lactic acid) (PLA) nanoparticles (PPNs) were used as the inner cores and 4T1 cancer cell membranes were coated on the surface of PPNs as the outer shells. The biomimetic platform was noted as CPPNs. The CPPNs exhibited proper sizes for the enhanced permeability and retention (EPR) effect and could maintain stability in a simulated physiological environment. The CPPNs exhibited a better antitumor effect than PPNs and free PTX in vitro. Moreover, due to the immune escape and homologous targeting abilities endowed by the cancer cell membrane coating, the CPPNs could efficiently accumulate and long-term exist at tumor sites. In the orthotopic 4T1 breast cancer mouse model, the CPPNs effectively inhibited the progression of tumor by increasing the apoptosis and necrosis areas within tumor tissues. In addition, the toxic side effects of PTX was also alleviated in the CPPNs group. As a result, CPPNs can be a promising biomimetic nanodrug delivery system for the enhanced and targeted therapy of breast cancer.

肿瘤部位的长循环时间和靶向药物递送仍然是纳米药物递送系统抗肿瘤活性的主要挑战。在此，制备了癌细胞膜包被的仿生纳米药物递送系统。以载有紫杉醇 (PTX) 的聚乳酸 (PLA) 纳米粒子 (PPNs) 为内核，将 4T1 癌细胞膜涂覆在 PPNs 的表面作为外壳。仿生平台被称为CPPN。CPPNs 表现出适当的尺寸以增强渗透性和保留 (EPR) 效果，并且可以在模拟生理环境中保持稳定性。CPPNs在体外表现出比 PPNs 和游离 PTX 更好的抗肿瘤作用. 此外，由于癌细胞膜涂层赋予的免疫逃逸和同源靶向能力，CPPNs可以在肿瘤部位有效积累并长期存在。在原位 4T1 乳腺癌小鼠模型中，CPPNs 通过增加肿瘤组织内的细胞凋亡和坏死区域有效地抑制了肿瘤的进展。此外，PTX 的毒副作用在 CPPNs 组中也得到了缓解。因此，CPPNs 可以成为一种很有前途的仿生纳米药物递送系统，用于乳腺癌的增强和靶向治疗。