The increasing demand for organs for transplantation necessitates the development of substitutes to meet the structural and physiological functions. Tissue decellularization and recellularization aids in retaining the three-dimensional integrity, biochemical composition, tissue ultra-structure, and mechanical behavior, which makes them functionally suitable for organ transplantation. Herein, we attempted to rebuild functional liver grafts in small animal model (Wistar rat) with a potential of translation. A soft approach was adopted using 0.1% SDS (Sodium Dodecyl Sulfate) for decellularization and primary hepatocytes were used as a potential cell source for recellularization. The decellularization process was evaluated and confirmed using histology, DNA content, ultra-structure analysis. The resultant scaffold was re-seeded with the rat hepatocytes and their biocompatibility was assessed by its metabolic functions and gene expression. The structural components of the Extracellular matrix (ECM) (Laminins, Collagen type I, Reticulins) were conserved and the liver cell-specific proteins like CK-18, alpha-fetoprotein, albumin were expressed in the recellularized scaffold. The functionality and metabolic activity of the repopulated scaffold were evident from the albumin and urea production. Expression of Cytokeratin-19 (CK-19), Glucose 6-Phosphatase (G6P), Albumin, Gamma Glutamyl Transferase (GGT) genes has distinctly confirmed the translational signals after the repopulation process. Our study clearly elucidates that the native extracellular matrix of rat liver can be utilized as a scaffold for effective recellularization for whole organ regeneration.

对移植器官的需求不断增加，需要开发替代品以满足结构和生理功能。组织脱细胞和再细胞化有助于保持三维完整性、生化成分、组织超微结构和机械行为，这使得它们在功能上适合器官移植。在此，我们尝试在具有翻译潜力的小动物模型（Wistar 大鼠）中重建功能性肝移植物。采用一种软方法，使用 0.1% SDS（十二烷基硫酸钠）进行脱细胞，并将原代肝细胞用作再细胞化的潜在细胞来源。使用组织学、DNA 含量、超微结构分析来评估和确认脱细胞过程。用大鼠肝细胞重新接种所得支架，并通过其代谢功能和基因表达评估其生物相容性。细胞外基质 (ECM) 的结构成分（层粘连蛋白、I 型胶原蛋白、网状蛋白）是保守的，肝细胞特异性蛋白质如 CK-18、甲胎蛋白、白蛋白在再细胞化支架中表达。从白蛋白和尿素的生产中可以明显看出重新填充的支架的功能和代谢活性。Cytokeratin-19 (CK-19)、葡萄糖 6-磷酸酶 (G6P)、白蛋白、γ-谷氨酰转移酶 (GGT) 基因的表达清楚地证实了再增殖过程后的翻译信号。