Fusarium guttiforme and Fusarium ananatum are the etiological agents of fusariosis and fruitlet core rot in pineapple, respectively, producing mycotoxins that are harmful to the health of consumers. These two fungi are morphologically similar and difficulty in obtaining macroconidia of the species limits their identification. Different types of media are available for the culture of these pathogens, but not all of them favor F. ananatum and F. guttiforme macroconidia production. Therefore, the objective of this study was to develop a simple culture medium to improve rapid macro- and microconidia formation in both F. guttiforme and F. ananatum to facilitate taxonomic, pathogenicity and mycotoxin studies. In vitro analysis showed that basal medium with carboxymethyl cellulose (CMC) was better than other media tested with the highest macroconidia production at 7 days of incubation. The highest production of microconidia was with synthetic nutrient medium (SN) at 7 days. F. ananatum produced a relatively high number of microconidia with one septum in comparison to F. guttiforme when cultured in CMC, which suggests an additional character useful for Fusarium taxonomy. CMC medium may serve as an improved alternative to culture media currently used in Fusarium research and contribute to further knowledge of the taxonomy and mycotoxins of Fusarium species.

中文翻译：

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用于提高分生孢子产量的培养基，用于鉴定和系统研究镰刀菌病原体。

镰刀形镰刀菌和凤梨镰刀菌分别是菠萝中的镰刀菌病和水果核腐烂的病原体，会产生对消费者健康有害的霉菌毒素。这两种真菌在形态上相似，难以获得该种的大分生孢子，限制了它们的鉴定。可以使用不同类型的培养基来培养这些病原体，但并非所有培养基都有利于花烛镰刀菌和古氏肠杆菌大分生孢子的产生。因此，本研究的目的是开发一种简单的培养基，以改善古氏肠杆菌和花环镰刀菌的快速大分生孢子和微分生孢子形成，以促进分类学，致病性和霉菌毒素的研究。体外分析表明，含羧甲基纤维素（CMC）的基础培养基在培养7天时比其他测试的最大分生孢子菌产量最高的培养基更好。微型分生孢子的最高产量是在7天使用合成营养培养基（SN）。与在CMC中培养的古菌F. ananatum相比，F。ananatum产生了相对较多的带有一个隔片的微分生孢子，这暗示了对镰刀菌分类有用的其他特征。CMC培养基可以替代目前镰刀菌研究中使用的培养基，并有助于进一步了解镰刀菌属的分类学和霉菌毒素。与在CMC中培养的古氏肠杆菌相比，凤梨产生了一个隔膜的相对较高数量的微分生孢子，这暗示了对镰刀菌分类有用的其他特征。CMC培养基可以替代目前镰刀菌研究中使用的培养基，并有助于进一步了解镰刀菌属的分类学和霉菌毒素。与在CMC中培养的古氏肠杆菌相比，凤梨产生了一个隔膜的相对较高数量的微分生孢子，这暗示了对镰刀菌分类有用的其他特征。CMC培养基可以替代目前镰刀菌研究中使用的培养基，并有助于进一步了解镰刀菌属的分类学和霉菌毒素。