A reverse genetics technology is an incredibly useful technique both for a proper understanding of different aspects of virus biology and for the generation of complementary DNA (cDNA)‐derived infectious viruses, which can act as safe and effective vaccines and viral vectors. Rotaviruses (RVAs), especially human RVAs (HuRVAs), had been very refractory to this technology until very recently. Here, we describe the historical background of the development of a long‐awaited HuRVA reverse genetics system, culminating in the generation of replicative HuRVAs entirely from cloned cDNAs.

中文翻译：

---

人类轮状病毒的反向遗传学系统。

逆向遗传学技术对于正确理解病毒生物学的各个方面以及生成互补DNA（cDNA）衍生的传染性病毒都是非常有用的技术，它们可以用作安全有效的疫苗和病毒载体。轮状病毒（RVA），尤其是人类RVA（HuRVAs），直到最近才对这种技术非常顽固。在这里，我们描述了期待已久的HuRVA逆向遗传系统发展的历史背景，最终产生了完全从克隆的cDNA复制出的HuRVAs。