Polyethylene glycol has been widely investigated for wound healing and dressing applications. Despite its advantages (i.e. great biocompatibility), polyethylene glycol lacks antibacterial activity. For this reason, semi-interpenetrated polymeric networks were prepared by combining a chemically cross-linked polyethylene glycol network with chitosan. The aim of this work was to identify the best amount of chitosan able to improve the antibacterial properties against Staphylococcus aureus. Briefly, the networks were synthesized by a sequential method, adding chitosan in different proportion to the polyethylene glycol. The antibacterial activity was tested following the MGA 0100 of the Pharmacopeia of the United States of Mexico. Fourier-transform infrared with attenuated total reflection spectroscopy, scanning electron microscopy and swelling behavior PBS at 37° C and room temperature were also performed to characterize the polymeric networks. The results showed that PC-2% was able to inhibit the bacterial growth of Staphylococcus aureus even more than Fosfomycin antibiotic. The networks showed cylindrical pores of different sizes (50–100 µm). The maximum swelling of all the networks was achieved in PBS at 37°C (>315%). Free hemoglobin and hemolysis assays were also evaluated to know the compatibility with erythrocytes. Human dermal fibroblasts were used to evaluate direct cytotoxicity. Therefore, the produced gels exerted interesting antibacterial activity and showed good biocompatibility properties.

聚乙二醇已被广泛研究用于伤口愈合和敷料应用。尽管有其优点（即良好的生物相容性），聚乙二醇缺乏抗菌活性。为此，通过将化学交联的聚乙二醇网络与壳聚糖结合来制备半互穿聚合物网络。这项工作的目的是确定能够提高对金黄色葡萄球菌的抗菌性能的最佳壳聚糖量. 简而言之，网络是通过顺序方法合成的，将不同比例的壳聚糖添加到聚乙二醇中。根据墨西哥药典的 MGA 0100 测试抗菌活性。还进行了带衰减全反射光谱的傅里叶变换红外光谱、扫描电子显微镜和 PBS 在 37°C 和室温下的溶胀行为，以表征聚合物网络。结果表明PC-2%能够抑制金黄色葡萄球菌的细菌生长甚至超过磷霉素抗生素。网络显示出不同尺寸（50-100 µm）的圆柱孔。所有网络的最大膨胀是在 37°C (>315%) 的 PBS 中实现的。还评估了游离血红蛋白和溶血测定以了解与红细胞的相容性。人真皮成纤维细胞用于评估直接细胞毒性。因此，生产的凝胶发挥了有趣的抗菌活性，并显示出良好的生物相容性。