Chronic hepatitis C virus (HCV) infection leads to variable outcomes, ranging from prolonged slow hepatic damage leading to cirrhosis, and hepatocellular carcinoma (HCC). Polymorphism in cytokines IL-10 and IL-12 that impact the immune response to HCV infection may play a role in determining this outcome. This study was aimed to determine if polymorphisms in IL-10 and IL-12B contribute to HCV susceptibility and the risk of developing HCC in patients from Northeast India. IL-10 − 1082, −819, −592 polymorphisms and IL-12B −1188 polymorphisms were genotyped by polymerase chain reaction–restriction fragment length polymorphism in a total of 266 HCV-infected patients and 100 age- and sex-matched controls. In the HCV-infected subjects, 110 patients had chronic hepatitis C (CHC), 96 with liver cirrhosis, and 60 with HCC. Serum levels of IL-10 were also measured and correlated with disease severity. Haplotype analysis for IL-10 polymorphisms was carried out. Statistical data were analyzed using SPSS ver. 22.0. The frequency of IL-10 − 592 AA genotype/A allele was significantly higher in HCC patients than in CHC patients. The intermediate IL-10-producing ACC haplotype was significantly more frequent in HCC and cirrhotic patients than in CHC patients. No significant association was found for IL-10 − 819, −592 and IL-12B −1188 polymorphisms with the susceptibility to HCV infection or occurrence of HCC in HCV-infected patients. IL-10 − 592 CA polymorphism and IL-10 ACC haplotype are significant biomarkers of HCC in HCV-infected patients from Northeast India. Higher serum levels of IL-10 were also linked to higher disease severity.

中文翻译：

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IL-10多态性和单倍型可预测印度东北部丙型肝炎病毒感染患者对肝细胞癌的易感性。

慢性丙型肝炎病毒（HCV）感染会导致不同的结果，其范围包括长期缓慢的肝损伤导致肝硬化和肝细胞癌（HCC）。影响对HCV感染的免疫反应的细胞因子IL-10和IL-12的多态性可能在确定这一结局中起作用。这项研究旨在确定IL-10和IL-12B中的多态性是否有助于HCV易感性以及印度东北部患者发展HCC的风险。IL-  10-1082，-819，-592多态性和IL-12B通过聚合酶链反应-限制性片段长度多态性对总共266例HCV感染的患者和100个年龄和性别匹配的对照组进行了-1188个多态性的基因分型。在感染HCV的受试者中，有110例患有慢性丙型肝炎（CHC），96例肝硬化和60例HCC。还测量了血清IL-10水平并将其与疾病严重程度相关。对IL-10多态性进行单倍型分析。统计数据使用SPSS ver。22.0。IL-10的频率 − HCC患者的592 AA基因型/ A等位基因显着高于CHC患者。与CHC患者相比，HCC和肝硬化患者中产生IL-10的中间单倍型更为频繁。没有发现IL- 10-819  ，-592和IL-12B -1188多态性与HCV感染患者易感染HCV或发生HCC有关。IL-  10-592 CA多态性和IL-10 ACC单倍型是印度东北地区HCV感染患者中HCC的重要生物标志物。较高的IL-10血清水平也与较高的疾病严重程度有关。