Abstract

Inhibitory activity of the biocontrol bacterial strain Lysobacter capsici AZ78 is related to the production of cyclo(l-Pro-l-Tyr), a 2,5-diketopiperazine with in vitro and in vivo toxic activity against Phytophthora infestans and Plasmopara viticola. Further investigation of culture filtrate organic extracts showed its ability to produce other 2,5-diketopiperazines. They were isolated and identified by spectroscopic (¹H NMR and ESIMS) and physic (specific optical rotation) methods as cyclo(l-Pro-l-Val), cyclo(d-Pro-d-Phe), cyclo(l-Pro-l-Leu), and cyclo(d-Pro-l-Tyr). When tested against the phytopathogenic Gram-positive bacterium Rhodococcus fascians LMG 3605, cyclo(l-Pro-l-Val) showed a toxic activity similar to chloramphenicol at a comparable concentration. Overall, these data suggest that 2,5-diketopiperazines represent a class of metabolites characterizing the metabolome of L. capsici AZ78. Furthermore, the toxic activity showed by cyclo(l-Pro-l-Val) against R. fascians LMG 3605 broaden the spectrum activity of 2,5-diketopiperazines against phytopathogenic microorganisms enforcing their potential development as biopesticides.

Isolation of 2,5-diketopiperazines from Lysobacter capsici AZ78 with activity against Rhodococcus fasciansAll authorsAlessio Cimmino ,Ana Bejarano ,Marco Masi ,Gerardo Puopolo &Antonio Evidente https://doi.org/10.1080/14786419.2020.1756803

Published online:

30 April 2020

中文翻译：

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从辣椒溶杆菌 AZ78 中分离出具有抗筋膜红球菌活性的 2,5-二酮哌嗪

摘要

生物防治细菌菌株Lysobacter capsici AZ78 的抑制活性与环 ( l -Pro- l -Tyr) 的产生有关，这是一种 2,5-二酮哌嗪，在体外和体内对致病疫霉和葡萄球菌具有毒性。对培养物滤液有机提取物的进一步研究表明其能够产生其他 2,5-二酮哌嗪。它们通过光谱（¹ H NMR 和 ESIMS）和物理（比旋光度）方法分离和鉴定为环（l -Pro- l -Val）、环（d -Pro- d -Phe）、环（l -Pro- l -Leu）和环（d -Pro- l -Tyr）。当针对致植物病原革兰氏阳性菌Rhodococcus fascians LMG 3605 进行测试时，环 ( l -Pro- l -Val) 在可比浓度下显示出与氯霉素相似的毒性活性。总体而言，这些数据表明，2,5二酮哌嗪代表了一类代谢物特征的代谢组L.辣椒AZ78。此外，cyclo( l -Pro- l -Val) 对R. fascians显示出毒性活性LMG 3605 拓宽了 2,5-二酮哌嗪对植物病原微生物的活性谱，增强了它们作为生物农药的潜力。

从辣椒溶杆菌 AZ78 中分离出具有抗筋膜红球菌活性的 2,5-二酮哌嗪所有作者Alessio Cimmino 、Ana Bejarano 、Marco Masi 、Gerardo Puopolo 和Antonio Evidente https://doi.org/10.1080/14720620419.

在线发布：

2020 年 4 月 30 日