The present study aims to employ a multiplex PCR-based method for phylogenetic typing of Shigella and determine the frequency of several virulence genes among Shigella phylogenetic clades and species. Species identification, phylogenetic typing of 44 previously diagnosed Shigella isolates, and frequency of virulence genes and loci, virA, virB, virF, ipaBCD, ial, sen, and set1A were investigated through performing several PCR assays. Distribution of virulence genes among Shigella phylogenetic clades and species was determined by the statistical analysis. The identities of 40 isolates out of 44 were confirmed as Shigella, and these isolates were classified in four phylogenetic clades, S1 (7.5%), S2 (52.5%), S3 (20%), and S5 (20%) and 4 species, S. sonnei (52.5%), S. flexneri (22.5%), S. dysenteriae (20%), and S. boydii (5%). The prevalence of virA, virB, virF, ipaBCD, ial, sen, and set1A was determined as 67.5%, 72.5%, 72.5%, 65%, 75%, 40%, and 5%, respectively. The presence of sen, uidA, or set1A was found to be statistically correlated with either of Shigella phylogenetic clades or species. A significant statistically association was also determined between set1A and Shigella phylogenetic clades. Furthermore, the nucleotide sequence of invasion-associated locus (ial) was determined and mapped on Shigella genome through in silico analysis. The current study shows the distribution of Shigella isolates and its key virulence genes within the five recently described phylogenetic clades for the first time in the Asia. This is also the first description of ial nucleotide sequence and its exact location on Shigella genome after its initial identification.

本研究旨在采用基于多重PCR的方法对志贺氏菌进行系统发育分型，并确定志贺氏菌系统进化枝和物种中几种毒力基因的频率。物种鉴定，44个先前诊断系统发育打字志贺氏菌分离株，和毒力基因和基因座的频率的virA，VIRB，virF，ipaBCD，IAL，SEN，和set1A是通过执行若干PCR测定的影响。志贺氏菌中毒力基因的分布通过统计分析确定系统进化枝和种类。确定了44个菌株中的40个菌株的身份为志贺氏菌（Shigella），并将这些菌株分为四个系统进化进化枝，S1（7.5％），S2（52.5％），S3（20％）和S5（20％）和4种，S。sonnei（52.5％），S。flexneri（22.5％），D。sensenteriae（20％）和S.boydii（5％）。的患病率的virA，VIRB，virF，ipaBCD，IAL，SEN，和set1A被确定为分别为67.5％，72.5％，72.5％，65％，75％，40％和5％。sen，uidA的存在，或set1A被发现与志贺氏菌系统进化分支或物种具有统计学相关性。在set1A和志贺氏菌系统进化进化枝之间也确定了显着的统计学关联。此外，通过计算机分析确定入侵相关基因座（ial）的核苷酸序列并将其定位在志贺氏菌基因组上。目前的研究表明，志贺氏菌分离物及其关键毒力基因在亚洲最近首次描述的五个进化系统进化枝中的分布。这也是对ial核苷酸序列及其确切位置的首次描述。志贺菌基因组经过初步鉴定。