Triatoma infestans (Klug) (Hemiptera: Reduviidae) is the main vector of Chagas disease in the Southern Cone of America and resistance to pyrethroid insecticides has been detected in several areas from its geographical distribution. Pyrethroid resistance presents a complex geographical pattern at different spatial scales. However, it is still unknown if the toxicological variability is a common feature within villages of the Gran Chaco were high resistance was descripted. The objectives of this study were to determine: (a) the microgeographical distribution of the deltamethrin-resistance in insects from Pampa Argentina village, (b) the performance of the insecticide impregnated paper bioassay to evaluate deltamethrin-resistance in field collected insects and (c) the lethal activity of the fumigant canister containing DDVP against insects resistant to deltamethrin. High survival of T. infestans exposed to discriminant dose was observed in the samples of all the evaluated dwellings, suggesting that the resistance to deltamethrin is homogeneous at the microgeographical level. Resistance determination by impregnated paper bioassay was similar to traditional topical determination, highlighting the use of this rapid methodology in field large-scale monitoring. The fumigant canister was not effective against resistant insects, remarking the need to develop suitable formulations that ensure minimal toxicological risk and high effectivity.

中文翻译：

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Triatoma infestans 对溴氰菊酯的抗性：微观地理分布、快速检测生物测定的验证和熏蒸剂罐作为控制替代策略的评估

三叶虫(Klug) (Hemiptera: Reduviidae) 是美洲南锥体锥虫病的主要传播媒介，从其地理分布来看，已在多个地区检测到对拟除虫菊酯杀虫剂的抗性。拟除虫菊酯耐药性在不同空间尺度上呈现出复杂的地理格局。然而，目前尚不清楚毒理学变异是否是格兰查科村庄内的共同特征，是否描述了高抗性。本研究的目的是确定：(a) 阿根廷潘帕村昆虫中溴氰菊酯抗性的微观地理分布，(b) 杀虫剂浸渍纸生物测定在田间采集昆虫中评估溴氰菊酯抗性的性能和 (c ) 含有 DDVP 的熏蒸剂罐对溴氰菊酯有抗药性的昆虫的致死活性。T. infestans在所有评估住宅的样本中观察到暴露于判别剂量，这表明对溴氰菊酯的抗性在微观地理水平上是均匀的。通过浸渍纸生物测定法测定耐药性与传统的局部测定相似，突出了这种快速方法在现场大规模监测中的应用。熏蒸剂罐对抗药性昆虫无效，这表明需要开发合适的配方以确保最小的毒理学风险和高效性。