A tissue engineered vessel has the potential to provide an alternative small diameter vascular graft for patients with cardiovascular disease in need of surgical revascularization. In this study, a polyglycolic acid (PGA) electrospun scaffold seeded with human dermal fibroblasts was stimulated with circumferential mechanical stretch by a pulsatile perfusion system. The PGA scaffold was fabricated using a custom electrospinning set-up to co-electrospray a sacrificial polyethylene oxide microparticle to increase pore size and bulk porosity. The tissue engineered vessel exposed to circumferential mechanical stretch was compared to an engineered vessel cultured under static conditions without any mechanical stimulation. The histology cross-sections demonstrated a similar thickness of engineered vessels with mechanical stretch and static, but on Masson’s Trichrome stain there was nearly twice the amount of staining for collagen. The collagen content was quantified, and the collagen c...

中文翻译：

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来自可生物降解的电纺支架的组织工程血管，受机械拉伸刺激。

组织工程血管有可能为需要手术血运重建的心血管疾病患者提供替代的小直径血管移植物。在这项研究中，聚乙醇酸 (PGA) 电纺支架接种了人类真皮成纤维细胞，通过脉冲灌注系统进行圆周机械拉伸刺激。PGA 支架是使用定制的静电纺丝装置制造的，以共电喷雾牺牲聚环氧乙烷微粒以增加孔径和体积孔隙率。将暴露于圆周机械拉伸的组织工程血管与在没有任何机械刺激的静态条件下培养的工程血管进行比较。组织学横截面显示具有相似厚度的工程血管具有机械拉伸和静态，但是在 Masson 的三色染色中，胶原蛋白的染色量几乎是其两倍。量化胶原蛋白含量，胶原蛋白...