MicroRNA dysregulation occurs in many human diseases, including atherosclerosis. Here, we examined the serum expression and clinical significance of miR-186-5p in patients with atherosclerosis, and explored its influence on vascular smooth muscle cell (VSMC) proliferation and migration. Blood samples were collected from 104 patients with asymptomatic atherosclerosis and 80 healthy controls. Quantitative real-time PCR was applied to measure the miR-186-5p level. An ROC curve was established to assess the discriminatory ability of the serum miR-186-5p level for identifying atherosclerosis from controls. CCK-8 and Transwell assays were used to evaluate the impact of miR-186-5p on cell behaviors. Serum expression of miR-186-5p was significantly higher in atherosclerosis patients than in the control group. The serum miR-186-5p level showed a positive correlation with CIMT and could be used to distinguish atherosclerosis patients from healthy controls, with an area under the curve (AUC) score of 0.891. In VSMCs, overexpression of miR-186-5p significantly promoted cell proliferation and migration, while the opposite results were observed when miR-186-5p was downregulated. Overexpression of miR-186-5p has a certain diagnostic significance for atherosclerosis. Upregulation of miR-186-5p stimulates VSMC proliferation and migration. Therefore, it is a possible target for atherosclerosis interventions.

中文翻译：

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MicroRNA-186-5p 作为动脉粥样硬化的诊断生物标志物并调节血管平滑肌细胞增殖和迁移。

MicroRNA 失调发生在许多人类疾病中，包括动脉粥样硬化。在这里，我们检测了 miR-186-5p 在动脉粥样硬化患者中的血清表达和临床意义，并探讨了其对血管平滑肌细胞 (VSMC) 增殖和迁移的影响。采集了 104 名无症状动脉粥样硬化患者和 80 名健康对照者的血样。应用定量实时 PCR 来测量 miR-186-5p 水平。建立 ROC 曲线以评估血清 miR-186-5p 水平对识别动脉粥样硬化与对照的区分能力。CCK-8 和 Transwell 分析用于评估 miR-186-5p 对细胞行为的影响。动脉粥样硬化患者血清中 miR-186-5p 的表达明显高于对照组。血清 miR-186-5p 水平与 CIMT 呈正相关，可用于区分动脉粥样硬化患者和健康对照，曲线下面积 (AUC) 评分为 0.891。在 VSMCs 中，miR-186-5p 的过表达显着促进细胞增殖和迁移，而当 miR-186-5p 下调时观察到相反的结果。miR-186-5p的过表达对动脉粥样硬化有一定的诊断意义。miR-186-5p 的上调刺激 VSMC 增殖和迁移。因此，它是动脉粥样硬化干预的可能目标。而当 miR-186-5p 被下调时观察到相反的结果。miR-186-5p的过表达对动脉粥样硬化有一定的诊断意义。miR-186-5p 的上调刺激 VSMC 增殖和迁移。因此，它是动脉粥样硬化干预的可能目标。而当 miR-186-5p 被下调时观察到相反的结果。miR-186-5p的过表达对动脉粥样硬化有一定的诊断意义。miR-186-5p 的上调刺激 VSMC 增殖和迁移。因此，它是动脉粥样硬化干预的可能目标。