AIM To compare the immunoexpression of RANK, MMP-9 and PTHrP in apical periodontitis lesions of diabetic and normoglycaemic individuals. METHODOLOGY Primary chronic apical periodontitis lesions associated with teeth indicated for extraction in 13 type 2 diabetic individuals and 13 normoglycaemic individuals who were screened for the glycaemic index and glycated haemoglobin (HbA1c) were analysed. Individuals with other systemic diseases and users of anti-inflammatories and/or antibiotics in the previous 3 months were excluded. Silanized slides with paraffin sections were used for immunohistochemical reactions and stained with haematoxylin and eosin for histopathological classification. The images were analysed with an optical microscope, and the slides were subdivided into five large fields assigning scores (0-2), according to the number of positive markings for each antibody. Fisher's exact test evaluated the parameters: gender, type of lesion, location and position in the arch. Nonparametric Mann-Whitney test was used for age, HbA1c values and comparison of marker expression. The chi-squared test was used to associate the expression of the markers. And the Spearman's coefficient correlated the markers with the size of the periapical lesion. RESULTS The samples consisted of 69% periapical granulomas and 31% periapical cysts in each group. RANK expression was considered weak/moderate and strong in, respectively, 62% and 38% of the cases in both groups. MMP-9 expression was weak/moderate and strong in, respectively, 38% and 62% of the cases from the diabetic group, in comparison with 38% and 38% in the normoglycaemics (24% cases from this group were negative). In contrast, PTHrP expression was negative, weak/moderate and strong in, respectively, 46%, 46% and 8% of the cases from the diabetic group, in comparison with 38% negative and 62% weak/moderate in normoglycaemics. Quantitative analysis revealed that there were no significant differences in the immunoexpression of RANK (P = 0.26), MMP-9 (P = 0.17) and PTHrP (P = 0.43) between the groups. There was no significant correlation between the expression of bone resorption markers and the macroscopic size of the periapical lesions (P > 0.05). CONCLUSIONS The bone resorption mediators analysed had similar immunoexpression in the periapical lesions of diabetic and normoglycaemic individuals.

中文翻译：

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糖尿病和正常血糖患者根尖周炎中骨吸收生物标志物的免疫表达。

目的比较RANK，MMP-9和PTHrP在糖尿病和血糖正常者的根尖周炎中的免疫表达。方法学分析了13名2型糖尿病患者和13名血糖正常患者的牙齿相关的原发性慢性根尖性牙周炎病变，这些患者经过了血糖指数和糖化血红蛋白（HbA1c）的筛查。排除了在过去3个月中患有其他全身性疾病的个人以及使用消炎药和/或抗生素的人。将具有石蜡切片的硅烷化载玻片用于免疫组织化学反应，并用苏木精和曙红染色以进行组织病理学分类。使用光学显微镜对图像进行分析，然后将幻灯片细分为五个大字段，分别指定得分（0-2），根据每种抗体的阳性标记数。Fisher的精确测试评估了以下参数：性别，病变类型，足弓位置和位置。非参数Mann-Whitney检验用于年龄，HbA1c值和标志物表达的比较。卡方检验用于关联标记的表达。Spearman系数使标志物与根尖周病变的大小相关。结果每组均由69％的根尖周肉芽肿和31％的根尖周囊肿组成。两组中分别有62％和38％的病例认为RANK表达弱/中度和强。在糖尿病组中，分别有38％和62％的患者MMP-9表达弱/中和强，相比之下，正常血糖组分别为38％和38％（该组24％的病例为阴性）。相反，在糖尿病组中，PTHrP表达分别为阴性，弱/中和强，分别为46％，46％和8％，而正常血糖组分别为38％的阴性和62％，弱/中。定量分析显示，两组之间RANK（P = 0.26），MMP-9（P = 0.17）和PTHrP（P = 0.43）的免疫表达没有显着差异。骨吸收标志物的表达与根尖周病变的宏观大小之间无显着相关性（P> 0.05）。结论所分析的骨吸收介质在糖尿病和血糖正常个体的根尖周病变中具有相似的免疫表达。糖尿病组分别有46％，46％和8％的患者处于弱/中度和强度状态，而正常血糖组中分别有38％的阴性/中度和62％的弱/中度情况。定量分析显示，两组之间RANK（P = 0.26），MMP-9（P = 0.17）和PTHrP（P = 0.43）的免疫表达没有显着差异。骨吸收标志物的表达与根尖周病变的宏观大小之间无显着相关性（P> 0.05）。结论所分析的骨吸收介质在糖尿病和血糖正常个体的根尖周病变中具有相似的免疫表达。糖尿病组分别有46％，46％和8％的患者处于弱/中度和强度状态，而正常血糖组分别为38％的阴性/中度和62％。定量分析显示，两组之间RANK（P = 0.26），MMP-9（P = 0.17）和PTHrP（P = 0.43）的免疫表达没有显着差异。骨吸收标志物的表达与根尖周病变的宏观大小之间无显着相关性（P> 0.05）。结论所分析的骨吸收介质在糖尿病和血糖正常个体的根尖周病变中具有相似的免疫表达。定量分析显示，两组之间RANK（P = 0.26），MMP-9（P = 0.17）和PTHrP（P = 0.43）的免疫表达没有显着差异。骨吸收标志物的表达与根尖周病变的宏观大小之间无显着相关性（P> 0.05）。结论所分析的骨吸收介质在糖尿病和血糖正常个体的根尖周病变中具有相似的免疫表达。定量分析显示，两组之间RANK（P = 0.26），MMP-9（P = 0.17）和PTHrP（P = 0.43）的免疫表达没有显着差异。骨吸收标志物的表达与根尖周病变的宏观大小之间无显着相关性（P> 0.05）。结论所分析的骨吸收介质在糖尿病和血糖正常个体的根尖周病变中具有相似的免疫表达。