A novel bilayer fibrous membrane for guided tissue regeneration (GTR) was prepared via two-step electrospinning process, subsequent crosslinking and surface conjugation with heparin. The bilayer membrane consists of upper layer polycaprolactone/gelatin (PCL/Gel) membrane for soft tissue regeneration and lower layer PCL/Gel/nano-hydroxyapatite (PCL/Gel/n-HA) membrane for hard tissue regeneration. The results indicated that the physicochemical and biological properties of the membrane were strongly influenced by the crosslinking time and by the heparin conjugation. Crosslinking effectively prolonged the degradation time while maintaining the membrane barrier function, and the surface heparin conjugation obviously improved the biological performance of the membrane. An enhanced cell adhesion and proliferation were observed on the heparin-conjugated fibrous membranes, which also showed good histocompatibility and favorable in vivo degradability. The electrospun bilayer fibrous membrane may have promising prospect for modulation of cell response and simultaneous regeneration of soft and hard tissues.

一种新型的双层用于引导组织再生纤维膜（GTR）的溶液通过两步静电纺丝工艺，随后与肝素交联和表面缀合。双层膜由用于软组织再生的上层聚己内酯/明胶 (PCL/Gel) 膜和用于硬组织再生的下层 PCL/Gel/纳米羟基磷灰石 (PCL/Gel/n-HA) 膜组成。结果表明，膜的理化和生物学特性受交联时间和肝素结合的强烈影响。交联在保持膜屏障功能的同时有效延长了降解时间，表面肝素偶联明显改善了膜的生物学性能。在肝素结合的纤维膜上观察到细胞粘附和增殖增强，这也显示出良好的组织相容性和有利的体内降解性。电纺双层纤维膜可能在调节细胞反应和软硬组织同步再生方面具有广阔的前景。