Mammalian pluripotent stem cells are thought to exist in two states: naive and primed. Generally, unlike those in rodents, pluripotent stem cells in primates, including humans, are regarded as being in the primed pluripotent state. Recently, several groups reported the existence of naive pluripotent stem cells in humans. In this study, we report the conversion of primed state embryonic stem cells from common marmoset, a New World monkey, to the naive state using transgenes. The cells showed typical naive state features, including dome-like colony morphology, growth factor requirement, gene expression profile, X chromosome activation state, and energy metabolic status. Moreover, interspecies chimeric embryo formation ability with mouse embryos was increased in the naive state. This technique can be applied in basic medical research using nonhuman primates, such as preclinical use of naive pluripotent stem cells and generating genetically modified primates.

中文翻译：

---

引发了普通Mar猴胚胎干细胞的天真转化。

哺乳动物的多能干细胞被认为以两种状态存在：天真和致敏。通常，与啮齿类动物不同，灵长类动物（包括人）中的多能干细胞被视为处于多能的初免状态。最近，几个小组报告了人类中幼稚多能干细胞的存在。在这项研究中，我们报告了使用转基因将初生状态的mar猴（一种新世界猴）从原始mar猴转化为幼稚状态的过程。这些细胞表现出典型的幼稚状态特征，包括圆顶状菌落形态，生长因子需求，基因表达谱，X染色体激活状态和能量代谢状态。此外，在幼稚状态下，种间嵌合小鼠胚胎形成能力增强。