Abstract

Satellite cells promote muscle repairing and muscle growth. Thereby the intention of the present study was to investigate the beneficial effects of heat stress at different time intervals on chicken satellite cells’ viability. Satellite cells were isolated from 1-day-old chicks and treated at two different temperatures (37 °C and 41 °C) for various time periods (6 h, 12 h, 24 h, 48 h, and 72 h). Both temperatures significantly increased cell viability after 24 h and 48 h. After 12 h, cell viability was significantly increased at 41 °C compared to 37 °C. However, more apoptotic cells were observed at end of the experiment of 41 °C compared to 37 °C. In addition, more live cells were found at early of experimental period at 41 °C than 37 °C. Additionally, protein and mRNA expression of HSP70, HP60 and HSP47 were significantly upregulated throughout the experimental period at temperature of 41 °C compared to those at 37 °C. These results indicate that cell viability and expression of heat stress related proteins/genes are induced by high temperature of 41 °C via heat stress pathway whereas activation of heat stress related proteins/genes are lower at 37 °C. Thus, 41 °C can trigger satellite cells’ viability essential for better cell survival than 37 °C at early incubation time.

摘要

卫星细胞促进肌肉修复和肌肉生长。因此，本研究的目的是研究不同时间间隔的热应激对鸡卫星细胞活力的有益影响。从 1 日龄雏鸡中分离出卫星细胞，并在两种不同温度（37 °C 和 41 °C）下处理不同时间段（6 小时、12 小时、24 小时、48 小时和 72 小时）。两种温度在 24 小时和 48 小时后都显着增加了细胞活力。12 小时后，与 37 °C 相比，41 °C 下的细胞活力显着增加。然而，与 37°C 相比，在 41°C 的实验结束时观察到更多的凋亡细胞。此外，在 41°C 的实验早期发现比 37°C 更多的活细胞。此外，HSP70 的蛋白质和 mRNA 表达，与 37 °C 相比，HP60 和 HSP47 在整个实验期间在 41 °C 温度下显着上调。这些结果表明，41°C 的高温通过热应激途径诱导细胞活力和热应激相关蛋白/基因的表达，而热应激相关蛋白/基因的激活在 37°C 时较低。因此，41 °C 可以触发卫星细胞的生存能力，这对于早期孵育时间比 37 °C 更好的细胞存活至关重要。