Annual Review of Genomics and Human Genetics ( IF 8.7 ) Pub Date : 2020-09-01 , DOI: 10.1146/annurev-genom-120219-083220 Guangdun Peng 1, 2, 3 , Guizhong Cui 2 , Jincan Ke 1 , Naihe Jing 1, 2, 3, 4
Embryonic development and stem cell differentiation provide a paradigm to understand the molecular regulation of coordinated cell fate determination and the architecture of tissue patterning. Emerging technologies such as single-cell RNA sequencing and spatial transcriptomics are opening new avenues to dissect cell organization, the divergence of morphological and molecular properties, and lineage allocation. Rapid advances in experimental and computational tools have enabled researchers to make many discoveries and revisit old hypotheses. In this review, we describe the use of single-cell RNA sequencing in studies of molecular trajectories and gene regulation networks for stem cell lineages, while highlighting the integratedexperimental and computational analysis of single-cell and spatial transcriptomes in the molecular annotation of tissue lineages and development during postimplantation gastrulation.
中文翻译:
在早期胚胎发育过程中,使用单细胞和空间转录组了解干细胞谱系规范。
胚胎发育和干细胞分化为理解协同细胞命运测定的分子调控和组织模式构建提供了范例。单细胞RNA测序和空间转录组学等新兴技术为解剖细胞组织,形态和分子特性的差异以及谱系分配开辟了新途径。实验和计算工具的飞速发展使研究人员能够进行许多发现并重新审视旧的假设。在这篇综述中,我们描述了单细胞RNA测序在干细胞谱系的分子轨迹和基因调控网络研究中的用途,