There is a great need for the progress of composite biomaterials, which are effective for tissue engineering applications. In this work, the development of composite electrospun nanofibres based on polycaprolactone (PCL) and collagen hydrolysate (CH) loaded with ferulic acid (FA) for the treatment of chronic wounds. Response Surface Methodology (RSM) has been applied to nanofibres factor manufacturing assisted by electrospinning. For wound healing applications, the authors have created the efficacy of CH, and PCL membranes can act as a stable, protective cover for wound, enabling continuous FA release. The findings of the RSM showed a reasonably good fit with a polynomial equation of the second order which was statistically acceptable at P < 0.05. The optimised parameters include the quantity of hydrolysate collagen, the voltage applied and the distance from tip-to-collector. Based on the Box-Behnken design, the RSM was used to create a mathematical model and optimise nanofibres with minimum diameter production conditions. Using FTIR, TGA and SEM, optimised nanofibres were defined. In vitro, cytocompatibility trials showed that there was an important cytocompatibility of the optimised nanofibres, which was proved by cell proliferation and cell morphology. In this research, the mixed nanofibres of PCL and CH with ferulic could be a potential biomaterial for wound healing.

中文翻译：

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用于组织工程应用的负载阿魏酸的胶原蛋白水解物和聚己内酯纳米纤维。

非常需要对组织工程应用有效的复合生物材料的进展。在这项工作中，开发了基于聚己内酯 (PCL) 和胶原蛋白水解物 (CH) 负载阿魏酸 (FA) 的复合电纺纳米纤维，用于治疗慢性伤口。响应面法 (RSM) 已应用于静电纺丝辅助的纳米纤维因子制造。对于伤口愈合应用，作者创造了 CH 的功效，PCL 膜可以作为伤口的稳定保护层，实现持续的 FA 释放。RSM 的结果显示出与二阶多项式方程的相当良好的拟合，这在 P < 0.05 时在统计学上是可接受的。优化的参数包括水解胶原蛋白的量，施加的电压和从尖端到集电极的距离。基于 Box-Behnken 设计，RSM 用于创建数学模型并优化具有最小直径生产条件的纳米纤维。使用 FTIR、TGA 和 SEM，定义了优化的纳米纤维。在体外，细胞相容性试验表明，优化后的纳米纤维具有重要的细胞相容性，这已通过细胞增殖和细胞形态得到证明。在这项研究中，PCL 和 CH 与阿魏酸的混合纳米纤维可能是用于伤口愈合的潜在生物材料。细胞相容性试验表明，优化后的纳米纤维具有重要的细胞相容性，细胞增殖和细胞形态证明了这一点。在这项研究中，PCL 和 CH 与阿魏酸的混合纳米纤维可能是用于伤口愈合的潜在生物材料。细胞相容性试验表明，优化后的纳米纤维具有重要的细胞相容性，细胞增殖和细胞形态证明了这一点。在这项研究中，PCL 和 CH 与阿魏酸的混合纳米纤维可能是用于伤口愈合的潜在生物材料。