In the present work, the preparation, characterisation, and efficiency of two different silica nanostructures as release vehicles of Cisplatin are reported. The 1-hexadeciltrimethyl-ammonium bromide templating agent was used to obtain mesoporous silica nanoparticles which were later loaded with Cisplatin. While sol-gel silica was very fast prepared using an excess of acetic acid during the hydrolysis-condensation reactions of tetraethylorthosilicate and at the same time the Cisplatin was added. Several physicochemical techniques including spectroscopies, electronic microscopy, X-ray diffraction, N2 adsorption-desorption were used to characterise the silica nanostructures. An in vitro Cisplatin release test was carried out using artificial cerebrospinal fluid. Finally, the toxicity of all silica nanostructures was tested using the C6 cancer cell line. The spectroscopic results showed the suitable stabilisation of Cisplatin into the two different silica nanostructures. A large surface area was obtained for the mesoporous silica nanoparticles, while low areas were obtained in the silica nanoparticles. Cisplatin was released faster from mesoporous silica channels than from inside of aggregates nanoparticles silica. Cisplatin alone, as well as, cisplatin released from both silica nanostructures exerted a toxic effect on cancer cells. In contrast, both silica structures without the drug did not exert any toxic effect.

中文翻译：

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用于在癌脑细胞上释放顺铂的二氧化硅基纳米颗粒的制备和表征。


在目前的工作中，报道了两种不同的二氧化硅纳米结构作为顺铂释放载体的制备、表征和效率。使用1-十六烷基三甲基溴化铵模板剂获得介孔二氧化硅纳米颗粒，然后将其负载顺铂。而在原硅酸四乙酯的水解-缩合反应过程中使用过量的乙酸并且同时添加顺铂，可以非常快速地制备溶胶-凝胶二氧化硅。使用光谱学、电子显微镜、X射线衍射、N2吸附-解吸等多种物理化学技术来表征二氧化硅纳米结构。使用人工脑脊液进行体外顺铂释放试验。最后，使用 C6 癌细胞系测试了所有二氧化硅纳米结构的毒性。光谱结果表明顺铂在两种不同的二氧化硅纳米结构中具有适当的稳定性。介孔二氧化硅纳米粒子获得了大的表面积，而二氧化硅纳米粒子获得了低的表面积。顺铂从介孔二氧化硅通道中的释放速度比从聚集体纳米颗粒二氧化硅内部的释放速度更快。单独的顺铂以及从二氧化硅纳米结构中释放的顺铂对癌细胞产生毒性作用。相比之下，不含药物的两种二氧化硅结构均未产生任何毒性作用。