Background

Serum triglyceride concentrations are measured as total glycerides content in many Western countries. In Japan, glycerol-blanked triglycerides (TG-GB) are measured to identify postprandial hypertriglyceridaemia and to minimize the influence of glycerol formulation on serum triglyceride values. However, TG-GB measurements have not been standardized. Therefore, we developed an efficient quantification system for total glycerides and free glycerol that allows the calculation of TG-GB concentrations.

Methods

We measured total glycerides and free glycerol in human serum by using gas chromatography-isotope dilution mass spectrometry and compared its performance to the reference method of the US Centers for Disease Control and Prevention (CDC).

Results

Our practical method of total glycerides and free glycerol quantification achieved excellent precision for both within-run and among-run coefficients of variation (<1.5% and <2.7%, respectively), with an average recovery of 99.8% for free glycerol. However, we noted an average %bias of –0.26% for total glycerides and –3.15% for free glycerol between our TG-GB method and the CDC reference method.

Conclusions

This practical method of total glycerides and free glycerol quantification enables traceability assessment of TG-GB measurements. Differences between the output values of TG-GB and the CDC reference method might result from the differences in free glycerol values.

中文翻译：

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一种通过气相色谱-同位素稀释质谱法测量甘油-空白甘油三酸酯浓度的方法。

背景

在许多西方国家中，血清甘油三酸酯浓度以甘油三酸酯的总含量衡量。在日本，测量甘油去皮的甘油三酸酯（TG-GB）可以识别餐后高甘油三酸酯血症，并使甘油制剂对血清甘油三酸酯值的影响最小化。但是，TG-GB测量尚未标准化。因此，我们开发了一种有效的总甘油酯和游离甘油定量系统，可以计算TG-GB浓度。

方法

我们通过使用气相色谱-同位素稀释质谱法测量了人血清中的总甘油酯和游离甘油，并将其性能与美国疾病控制与预防中心（CDC）的参考方法进行了比较。

结果

我们实用的总甘油酯和游离甘油定量方法在批内和批间变异系数（分别<1.5％和<2.7％）上均达到了极好的精度，游离甘油的平均回收率为99.8％。但是，我们注意到，在我们的TG-GB方法和CDC参考方法之间，甘油三酯的总平均偏差为–0.26％，游离甘油的平均偏差为–3.15％。

结论

这种实用的总甘油酯和游离甘油定量方法可以实现TG-GB测量的可追溯性评估。TG-GB的输出值和CDC参考方法之间的差异可能是由游离甘油值的差异引起的。