Background

Plasma viscosity is an important biomarker both in diagnostics and treatment monitoring of plasma cell dyscrasias and other disorders with hyperviscosity syndrome as a clinical manifestation. Here, we investigate the performance of a new microfluidic-based viscometer for clinical use, establish a new reference range to be used with this instrument and determine the importance of sampling temperature.

Methods

The microVisc™ viscometer was evaluated for within-run and between-run imprecision and bias using standardized reference material (Paragon controls) and Seronorm™ control material. The reference range was established for the adult population using EDTA-plasma from 120 healthy blood donors. Sampling temperature was investigated by drawing and transporting blood at room temperature and 37°C and comparing the viscosity between the two sampling methods.

Results

The microfluidic-based viscometer performed well, and imprecision was comparable to ReoRox® G2 free oscillation rheometer. A new reference range for the adult Danish population was established as 1.2–1.5 mPa s at 37°C. Furthermore, sampling temperature at room temperature and 37°C was investigated, and there was no difference in results obtained.

Conclusions

MicroVisc™ is suitable for measuring plasma viscosity in a clinical setting and results can be evaluated using the established reference range. Blood sampling for viscosity analysis can be performed as a standard procedure at room temperature.

中文翻译：

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血浆粘度：临床上使用微流体芯片技术（microVisc™）评估新的测量方法，并确定新的参考范围。

背景

血浆粘度是浆细胞发育不良和其他以高粘度综合征为临床表现的疾病的诊断和治疗监测的重要生物标志物。在这里，我们研究了一种新的基于微流体的粘度计在临床上的性能，建立了与该仪器一起使用的新参考范围，并确定了采样温度的重要性。

方法

使用标准参考材料（Paragon对照品）和Seronorm™对照品对microVisc™粘度计进行了批内和批间不精确度和偏倚评估。使用120名健康献血者的EDTA血浆为成年人群建立了参考范围。通过在室温和37°C下抽血和运输血液并比较两种采样方法之间的粘度来研究采样温度。

结果

基于微流体的粘度计性能良好，其精确度可与ReoRox®G2自由振荡流变仪媲美。在37°C下，丹麦成年人口的新参考范围确定为1.2–1.5 mPa s。另外，研究了室温和37℃下的采样温度，结果没有差异。

结论

MicroVisc™适用于在临床环境中测量血浆粘度，并且可以使用已建立的参考范围评估结果。用于粘度分析的血液采样可以在室温下作为标准程序执行。