The fur is hard to decompose during the fermentation process of diseased swine carcasses. In order to enhance the enzymolysis of pigskin, the ultrasonic was proposed to use during the process of the enzymatic hydrolysis. The response surface optimization experiments were carried out with the DH (degree of hydrolysis) as the response value and the optimum conditions for enzymatic hydrolysis were determined. Based the optimum conditions, orthogonal experiments were carried out with ultrasonic frequency, power and time as variables, and optimal ultrasonic parameters were obtained. Without the assistance of ultrasonic, the descending order of influence factors on DH was, temperature＞SC(Substrate concentration)＞RES(The ratio of enzyme to substrate)＞pH. Moreover, the DH value is of 10.42% under the following optimal conditions: RES is of 16,006 U/g, the temperature is of 48.92°C, the SC is of 59.76 g/L and pH is of 10.43. Frequency has the greatest effect on DH, followed by power, and finally time. The optimum hydrolysis time is of 5 h, and the DH is of 22.94% were obtained under the following optimum ultrasonic pretreatment conditions: frequency combination is of (20,40,40), power is of 600 W and time is of 25 min. Comparing with the group without ultrasonic pretreatment, the DH for the ultrasonic assistance increased by 4%, the hydrolysis time was shorten by 3 h, and the total amino acids increased by 15.98%.

中文翻译：

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超声波辅助增强猪皮的酶解作用。

在患病猪尸体的发酵过程中，皮毛很难分解。为了增强猪皮的酶解，有人提出在酶解过程中使用超声波。以DH（水解度）为响应值进行响应面优化实验，确定了酶促水解的最佳条件。根据最佳条件，以超声频率，功率和时间为变量进行正交试验，得到最佳超声参数。在没有超声波辅助的情况下，影响DH的因素从高到低依次为温度＞ SC（底物浓度）＞ RES（酶与底物的比例）＞ pH。此外，在以下最佳条件下，DH值为10.42％：RES为16,006 U / g，温度为48.92°C，SC为59.76 g / L，pH为10.43。频率对DH的影响最大，其次是功率，最后是时间。在以下最佳超声预处理条件下，最佳水解时间为5 h，DH为22.94％：频率组合为（20,40,40），功率为600 W，时间为25分钟。与未进行超声波预处理的组相比，用于超声波辅助的DH增加了4％，水解时间缩短了3 h，总氨基酸增加了15.98％。在以下最佳超声预处理条件下获得94％的频率：频率组合为（20,40,40），功率为600 W，时间为25分钟。与未进行超声波预处理的组相比，用于超声波辅助的DH增加了4％，水解时间缩短了3 h，总氨基酸增加了15.98％。在以下最佳超声预处理条件下获得94％的频率：频率组合为（20,40,40），功率为600 W，时间为25分钟。与未进行超声波预处理的组相比，用于超声波辅助的DH增加了4％，水解时间缩短了3 h，总氨基酸增加了15.98％。