Abstract In vitro activity against methicillin-resistant Staphylococcus aureus (MRSA) and Staphylococcus epidermidis biofilm producers from blood cultures of patients with prosthetic hip infections was evaluated. The Minimum Inhibitory Concentration (MIC) for AP7121 was determined and the bactericidal activity of AP7121 (MICx1, MICx4) against planktonic cells was studied at 4, 8 and 24 h. The biofilms formed were incubated with AP7121 (MICx1, MICx4) for 1 and 24 h. The anti-adhesion effect of an AP7121-treated inert surface over the highest MIC isolate was studied with scanning electron microscopy (SEM). The bactericidal activity of AP7121 against all the planktonic staphylococcal cells was observed at 4 h at both peptide concentrations. Dose-dependent anti-biofilm activity was detected. AP7121 (MICx4) showed bactericidal activity at 24 h in all isolates. SEM confirmed prevention of biofilm formation. This research showed the in vitro anti-biofilm activity of AP7121 against MRSA and S. epidermidis and the prevention of biofilm formation by them on an abiotic surface.

中文翻译：

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抗菌肽 AP7121 对人类耐甲氧西林生物膜生产者金黄色葡萄球菌和表皮葡萄球菌的体外活性

摘要 评估了对来自假体髋关节感染患者血培养物的耐甲氧西林金黄色葡萄球菌 (MRSA) 和表皮葡萄球菌生物膜生产者的体外活性。测定了 AP7121 的最小抑制浓度 (MIC)，并在 4、8 和 24 小时研究了 AP7121（MICx1、MICx4）对浮游细胞的杀菌活性。形成的生物膜与 AP7121 (MICx1, MICx4) 孵育 1 和 24 小时。用扫描电子显微镜 (SEM) 研究了 AP7121 处理的惰性表面对最高 MIC 分离物的抗粘附作用。在两种肽浓度下，在 4 小时观察到 AP7121 对所有浮游葡萄球菌细胞的杀菌活性。检测到剂量依赖性抗生物膜活性。AP7121 (MICx4) 在所有分离株中在 24 小时时显示出杀菌活性。SEM 证实可防止生物膜形成。该研究显示了 AP7121 对 MRSA 和表皮葡萄球菌的体外抗生物膜活性以及它们在非生物表面上防止生物膜形成的作用。