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Characterizing the Binding of Angiotensin Converting Enzyme I Inhibitory Peptide to Human Hemoglobin: Influence of Electromagnetic Fields.
Protein & Peptide Letters ( IF 1.0 ) Pub Date : 2020-09-30 , DOI: 10.2174/1871530320666200425203636
Farzaneh Sadeghzadeh 1 , Amir Arsalan Entezari 1 , Kiana Behzadian 1 , Kimia Habibi 1 , Zeinab Amiri-Tehranizadeh 2 , Ahmad Asoodeh 3 , Mohammad Reza Saberi 2 , Jamshidkhan Chamani 1
Affiliation  

Background: Drug-protein complexes is one of the crucial factors when analyzing the pharmacokinetics and pharmacodynamics of a drug because they can affect the excretion, distribution, metabolism and interaction with target tissues.

Objectives: The aim of this study was to investigate the interaction of human hemoglobin (Hb) and angiotensin I converting enzyme inhibitory peptide (ACEIP) in the absence and presence of different- frequency electromagnetic fields (EMF).

Methods: Various spectroscopic methods like fluorescence spectroscopy, ultraviolet, circular dichroism and conductometry techniques were applied to investigate Hb-ACEIP interaction in the absence and presence of EMF.

Result: The presented spectroscopic studies indicated that EMF changed the interaction between Hb and ACEIP. The a-helix content of Hb decreased upon binding to ACEIP and conductivity of the solution enhanced upon binding. Based on Stern-Volmer equations, it could be stated that the Hb-ACEIP affinity was higher in the presence of EMF.

Conclusion: It can be concluded that for patients who use the drug to control blood pressure, a low-frequency electromagnetic field would have a positive effect on the uptake of the drug.



中文翻译:

表征血管紧张素转化酶I抑制肽与人血红蛋白的结合:电磁场的影响。

背景:药物-蛋白质复合物是分析药物的药代动力学和药效学时的关键因素之一,因为它们会影响排泄,分布,代谢以及与靶组织的相互作用。

目的:本研究的目的是研究在不存在和存在不同频率电磁场(EMF)的情况下人血红蛋白(Hb)与血管紧张素I转化酶抑制肽(ACEIP)的相互作用。

方法:采用多种光谱方法,如荧光光谱法,紫外光谱,圆二色性和电导技术,研究在有无EMF的情况下Hb-ACEIP的相互作用。

结果:目前的光谱研究表明,EMF改变了Hb与ACEIP之间的相互作用。结合ACEIP后,Hb的α-螺旋含量降低,结合后溶液的电导率提高。基于Stern-Volmer方程,可以说在EMF存在下,Hb-ACEIP亲和力更高。

结论:可以得出结论,对于使用该药物控制血压的患者,低频电磁场将对该药物的吸收产生积极影响。

更新日期:2020-11-04
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