Background: Drug-protein complexes is one of the crucial factors when analyzing the pharmacokinetics and pharmacodynamics of a drug because they can affect the excretion, distribution, metabolism and interaction with target tissues.

Objectives: The aim of this study was to investigate the interaction of human hemoglobin (Hb) and angiotensin I converting enzyme inhibitory peptide (ACEIP) in the absence and presence of different- frequency electromagnetic fields (EMF).

Methods: Various spectroscopic methods like fluorescence spectroscopy, ultraviolet, circular dichroism and conductometry techniques were applied to investigate Hb-ACEIP interaction in the absence and presence of EMF.

Result: The presented spectroscopic studies indicated that EMF changed the interaction between Hb and ACEIP. The a-helix content of Hb decreased upon binding to ACEIP and conductivity of the solution enhanced upon binding. Based on Stern-Volmer equations, it could be stated that the Hb-ACEIP affinity was higher in the presence of EMF.

Conclusion: It can be concluded that for patients who use the drug to control blood pressure, a low-frequency electromagnetic field would have a positive effect on the uptake of the drug.

背景：药物-蛋白质复合物是分析药物的药代动力学和药效学时的关键因素之一，因为它们会影响排泄，分布，代谢以及与靶组织的相互作用。

目的：本研究的目的是研究在不存在和存在不同频率电磁场（EMF）的情况下人血红蛋白（Hb）与血管紧张素I转化酶抑制肽（ACEIP）的相互作用。

方法：采用多种光谱方法，如荧光光谱法，紫外光谱，圆二色性和电导技术，研究在有无EMF的情况下Hb-ACEIP的相互作用。

结果：目前的光谱研究表明，EMF改变了Hb与ACEIP之间的相互作用。结合ACEIP后，Hb的α-螺旋含量降低，结合后溶液的电导率提高。基于Stern-Volmer方程，可以说在EMF存在下，Hb-ACEIP亲和力更高。

结论：可以得出结论，对于使用该药物控制血压的患者，低频电磁场将对该药物的吸收产生积极影响。