The purpose of this study was to describe the prevalence and incidence of Neospora caninum infection in dogs that are in close contact with dairy cattle and to identify possible risk factors associated with the infection in this population. Twenty-four dogs located in 8 different dairy farms of Aguascalientes, Mexico, were evaluated for a 6-mo period. Once a month a sample of serum and a sample of peripheral blood was collected. The serum was used to detect antibodies against N. caninum by means of the indirect immunofluorescence technique, and the blood was used to detect parasite's DNA. The association between seroprevalence and possible risk factors was estimated using logistic regression. The prevalence of anti-N. caninum antibodies was 54% in the first month, 62% in the last month, and the incidence was 8.69%. One farm had no positive cases. Antibody titers ranged from 1:50 to 1:800. Parasite DNA was not detected in any of the samples. Only the age (>6 yr) of the dogs was identified as a risk factor for infection by N. caninum (P ≤ 0.05).

中文翻译：

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墨西哥中北部奶牛场犬新孢子虫感染的流行率，发病率和危险因素。

这项研究的目的是描述与奶牛密切接触的犬新孢子虫感染的发生率和发生率，并确定与该人群感染有关的可能危险因素。对位于墨西哥阿瓜斯卡连特斯8个不同奶牛场的24条狗进行了为期6个月的评估。每月一次收集血清样品和外周血样品。用间接免疫荧光技术将血清用于检测犬新孢子虫的抗体，并用血液检测寄生虫的DNA。血清流行率和可能的危险因素之间的关联使用逻辑回归进行估计。抗N的流行。第一个月的犬类抗体为54％，最后一个月为62％，发生率为8.69％。一个农场没有阳性病例。抗体效价范围为1:50至1：800。在任何样品中均未检测到寄生虫DNA。仅将狗的年龄（> 6岁）确定为犬新孢子虫感染的危险因素（P≤0.05）。