Neuroinflammation is a secondary response following ischemia stroke. Arginine is a non-essential amino acid that has been shown to inhibit acute inflammatory reaction. In this study we show that arginine treatment decreases neuronal death after rat cerebral ischemia/reperfusion (I/R) injury and improves functional recovery of stroke animals. We also show that arginine suppresses inflammatory response in the ischemic brain tissue and in the cultured microglia after OGD insult. We further provide evidence that the levels of HIF-1α and LDHA are increased after rat I/R injury and that arginine treatment prevents the elevation of HIF-1α and LDHA after I/R injury. Arginine inhibits inflammatory response through suppression of HIF-1α and LDHA in the rat ischemic brain tissue and in the cultured microglia following OGD insult, and protects against ischemic neuron death after rat I/R injury by attenuating HIF-1α/LDHA-mediated inflammatory response. Together, these results indicate a possibility that arginine-induced neuroprotective effect may be through the suppression of HIF-1α/LDHA-mediated inflammatory response in microglia after cerebral ischemia injury.

中文翻译：

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精氨酸通过抑制HIF-1α/ LDHA介导的脑缺血/再灌注损伤后的炎症反应而具有神经保护作用。

神经炎症是缺血性中风后的继发性反应。精氨酸是一种非必需氨基酸，已被证明可以抑制急性炎症反应。在这项研究中，我们表明精氨酸治疗可降低大鼠脑缺血/再灌注（I / R）损伤后的神经元死亡，并改善中风动物的功能恢复。我们还显示，精氨酸抑制OGD损伤后缺血性脑组织和培养的小胶质细胞的炎症反应。我们进一步提供的证据表明，大鼠I / R损伤后HIF-1α和LDHA的水平升高，精氨酸治疗可防止I / R损伤后HIF-1α和LDHA的升高。精氨酸通过抑制OGD损伤后大鼠缺血性脑组织和培养的小胶质细胞中的HIF-1α和LDHA来抑制炎症反应，并通过减弱HIF-1α/ LDHA介导的炎症反应来保护大鼠I / R损伤后缺血性神经元死亡。总之，这些结果表明精氨酸诱导的神经保护作用可能是通过抑制脑缺血后小胶质细胞中的HIF-1α/ LDHA介导的炎症反应而实现的。