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Transcriptomic analysis of long noncoding RNAs and mRNAs expression profiles in the spinal cord of bone cancer pain rats.
Molecular Brain ( IF 3.3 ) Pub Date : 2020-03-24 , DOI: 10.1186/s13041-020-00589-2
Xinran Hou 1 , Yingqi Weng 1 , Qulian Guo 1 , Zhuofeng Ding 1 , Jian Wang 1 , Jiajia Dai 1 , Anqi Wei 1 , Zongbin Song 1
Affiliation  

Bone cancer pain (BCP) is one of the most common types of chronic cancer pain and its pathogenesis has not been fully understood. Long non-coding RNAs (lncRNAs) are new promising targets in the field of pain research, however, their involvements in BCP have not been reported. In the present study, we established the BCP model by implantation of Walker 256 carcinoma cells into rats' tibial medullary cavity and performed transcriptome sequencing of the ipsilateral lumbar spinal cord to explore changes in expression profiles of lncRNA and mRNA. We identified 1220 differently expressed mRNAs (DEmRNAs) (1171 up-regulated and 49 down-regulated) and 323 differently expressed lncRNAs (DElncRNAs) (246 up-regulated and 77 down-regulated) in BCP model, among which 10 DEmRNAs (5 up-regulated and 5 down-regulated) and 10 DElncRNAs (5 up-regulated and 5 down-regulated) were validated the expression by RT-qPCR. Then, we performed Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis on the expression of DEmRNAs and DElncRNAs, showing that they were mainly enriched in inflammatory and immunologic processes/pathways. Finally, we constructed a co-expression network and a ceRNA network of DEmRNAs and DElncRNAs to exhibit a potential regulatory mechanism of DElncRNAs, directly regulating protein coding gene expression in cis or in trans and indirectly regulating protein coding gene expression by sponging miRNA. In conclusion, our study provided a landscape of dysregulated lncRNA and mRNA in spinal cord of bone cancer pain and detected novel potential targets for treatment in the future.

中文翻译:

转录组学分析骨癌疼痛大鼠脊髓中较长的非编码RNA和mRNA表达谱。

骨癌疼痛(BCP)是慢性癌疼痛的最常见类型之一,其发病机理尚未完全了解。长非编码RNA(lncRNA)是疼痛研究领域中新的有希望的靶标,但是,尚未报道它们参与BCP的研究。在本研究中,我们通过将Walker 256癌细胞植入大鼠胫骨髓腔中建立BCP模型,并对同侧腰脊髓进行转录组测序,以探索lncRNA和mRNA表达谱的变化。我们在BCP模型中鉴定了1220种不同表达的mRNA(DEmRNA)(1171上调和49种下调)和323种不同表达的lncRNA(DElncRNAs)(246种上调和77种下调),RT-qPCR验证了其中的10个DEmRNA(5个上调和5个下调)和10个DElncRNAs(5个上调和5个下调)的表达。然后,我们对DEmRNA和DElncRNA的表达进行了基因本体论(GO)和《京都基因与基因组百科全书》(KEGG)分析,表明它们主要在炎症和免疫过程/途径中富集。最后,我们构建了DEmRNA和DElncRNA的共表达网络和ceRNA网络,以展示DElncRNA的潜在调控机制,直接调控顺式或反式编码蛋白的基因表达,并通过纺成miRNA间接调节蛋白质编码的基因表达。结论,
更新日期:2020-03-24
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