A HPLC-DAD method was established for the simultaneous determination of eight components in Leonuri Herba from different habitats, as well as providing methodological reference for quality control of Leonuri Herba. In this study, absolute ethanol extracts of the medicinal material were considered as the sample solutions to be analyzed. Agilent ZORBAX SB-C18 column (250 mm × 4.6 mm, 5 μm) was used for determination maintained at the temperature of 30°C. Gradient elution was performed with a mobile phase of methanol (B)-0.1% phosphoric acid solution (A) at the flow rate of 1.0 mL·min-1. The analysis was carried out at the wavelength of 225, 280 and 320 nm. The measured eight components showed good linear relationships within their own concentration range. Average recoveries ranged from 98.83 to 100.36% with RSDs of 1.14~1.97%. The proposed method was found to be simple, accurate and reproducible, which provided an effective quantitative analytical method for quality control of Leonuri Herba from different habitats.

中文翻译：

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HPLC-DAD同时测定不同生境中益母草中的八种成分。

建立了HPLC-DAD方法，用于同时测定不同生境中益母草中的八种成分，并为益母草的质量控制提供方法学参考。在这项研究中，药用材料的无水乙醇提取物被认为是要分析的样品溶液。使用Agilent ZORBAX SB-C18色谱柱（250 mm×4.6 mm，5μm）保持在30°C的温度下进行测定。用甲醇（B）-0.1％磷酸溶液（A）的流动相以1.0 mL·min-1的流速进行梯度洗脱。分析是在225、280和320 nm的波长下进行的。测得的八种成分在其各自的浓度范围内显示出良好的线性关系。平均回收率在98.83至100.36％之间，RSD为1.14〜1.97％。