To study the genotoxic impacts of Sabal discharges, three sites around Sabal drain were selected and compared to a reference site (site1). Site2 was at the southern part of the main canal, site3 was at the main canal outlet, and site4 at the northern part of the main canal. Compared to the reference fish, the recorded micronuclei and other nuclear anomalies showed marked (p < 0.05) increases with different frequencies in the studied sites. The induction of nuclear deformations was as following site3 > site4 > site2 > site1. The analysis of comet assay data showed that the DNA damage (based on the percentage of tail DNA) was significantly increased and the levels of damage were associated with the distance from the main discharge point. Moreover, DNA damages showed variable percentages among the studied tissues. The gills and liver tissues collected from site3 showed the highest DNA damage compared with low muscular DNA damage.

中文翻译：

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长期暴露于Sa漏会引起对尼罗罗非鱼的遗传毒性作用。

为了研究Sabal排放物的遗传毒性影响，选择了Sabal排水管周围的三个地点，并将其与参考地点（site1）进行了比较。站点2在干渠的南部，站点3在干渠的出口，站点4在干渠的北部。与参考鱼相比，记录的微核和其他核异常显示出明显的（p <0.05）随研究地点的不同频率而增加。诱导核变形的顺序如下：site3> site4> site2> site1。彗星分析数据的分析表明，DNA损伤（基于尾部DNA的百分比）显着增加，并且损伤程度与距主要放电点的距离有关。此外，DNA损伤在研究的组织中显示出可变的百分比。与低肌肉DNA损伤相比，从site3收集的腮和肝组织显示出最高的DNA损伤。