当前位置: X-MOL 学术Transpl. Immunol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Gastric submucosal alleviated pro-inflammation cytokines mediated initial dysfunction of islets allografts.
Transplant Immunology ( IF 1.6 ) Pub Date : 2020-04-14 , DOI: 10.1016/j.trim.2020.101292
Zhuzeng Yin 1 , Jiaxin Li 2 , Yang Zheng 2 , Shusen Wang 3 , Ximo Wang 4
Affiliation  

BACKGROUND The liver and renal capsule are the most common site for experimental pancreatic islet transplantation, but it is not optimal. Gastric submucosa space may be an ideal site for islet transplantation; however, whether pro-inflammation factors mediated islet dysfunction could be avoided or alleviated is still unclear. METHODS Islets of Sprague Dawley (SD) rat were transplanted into the streptozotocin-induced diabetic SD rats. Transplantation sites included gastric submucosa (GS), intraportal vein (PV) and kidney capsule (KC), and the efficiency of glycemic control and site-specific differences of islet grafts were compared. RESULTS With limited number of islets (800 IEQ) transplanted, improvement of recipient glycometabolism was superior in the GS group. When transplanted with 1200 IEQ islets, the survival of islet grafts were significantly prolonged in the GS group (25.87 ± 4.08 days, compared to 15.97 ± 0.83 days and 17.33 ± 1.41 days in PV and KC groups, respectively, P < .05). Compared with the PV group, the levels of IL-1β and TNF-α were significantly depressed in GS group after 12 h transplantation (15.5 ± 0.70 pg/mL and 13.28 ± 2.80 pg/mL vs. 262.26 ± 53.37 pg/mL and 138.51 ± 39.58 pg/mL, P < .05). CONCLUSIONS Gastric submucosal would be a potential ideal site for islet transplantation in rat. Gastric submucosal might alleviate the early islet dysfunction triggered by the IL-1β and TNF-α, and which requires a low number of transplanted islets and have a good glycemic control in return.

中文翻译:

胃粘膜下层减轻促炎细胞因子介导的胰岛同种异体移植物的初始功能障碍。

背景 肝肾包膜是实验性胰岛移植最常见的部位,但并非最佳。胃黏膜下层空间可能是胰岛移植的理想部位;然而,是否可以避免或减轻促炎因子介导的胰岛功能障碍仍不清楚。方法将Sprague Dawley(SD)大鼠胰岛移植到链脲佐菌素诱导的糖尿病SD大鼠体内。移植部位包括胃黏膜下层(GS)、门静脉(PV)和肾包膜(KC),比较了胰岛移植物的血糖控制效率和部位特异性差异。结果 由于移植的胰岛数量有限(800 IEQ),GS 组的受体糖代谢改善更为明显。移植1200个IEQ胰岛时,GS 组胰岛移植物的存活率显着延长(25.87 ± 4.08 天,PV 组和 KC 组分别为 15.97 ± 0.83 天和 17.33 ± 1.41 天,P < .05)。与 PV 组相比,移植 12 h 后 GS 组 IL-1β 和 TNF-α 水平显着降低(15.5 ± 0.70 pg/mL 和 13.28 ± 2.80 pg/mL vs. 262.26 ± 53.37 pg/mL 和 138.51 ± 39.58 pg/mL,P < .05)。结论胃黏膜下层可能是大鼠胰岛移植的理想部位。胃黏膜下层可能会缓解由 IL-1β 和 TNF-α 引发的早期胰岛功能障碍,这需要较少的移植胰岛数量并具有良好的血糖控制作为回报。P < .05)。与 PV 组相比,移植 12 h 后 GS 组 IL-1β 和 TNF-α 水平显着降低(15.5 ± 0.70 pg/mL 和 13.28 ± 2.80 pg/mL vs. 262.26 ± 53.37 pg/mL 和 138.51 ± 39.58 pg/mL,P < .05)。结论胃黏膜下层可能是大鼠胰岛移植的理想部位。胃黏膜下层可能会缓解由 IL-1β 和 TNF-α 引发的早期胰岛功能障碍,这需要较少的移植胰岛数量并具有良好的血糖控制作为回报。P < .05)。与 PV 组相比,移植 12 h 后 GS 组 IL-1β 和 TNF-α 水平显着降低(15.5 ± 0.70 pg/mL 和 13.28 ± 2.80 pg/mL vs. 262.26 ± 53.37 pg/mL 和 138.51 ± 39.58 pg/mL,P < .05)。结论胃黏膜下层可能是大鼠胰岛移植的理想部位。胃黏膜下层可能会缓解由 IL-1β 和 TNF-α 引发的早期胰岛功能障碍,这需要较少的移植胰岛数量并具有良好的血糖控制作为回报。结论胃黏膜下层可能是大鼠胰岛移植的理想部位。胃黏膜下层可能会缓解由 IL-1β 和 TNF-α 引发的早期胰岛功能障碍,这需要较少的移植胰岛数量并具有良好的血糖控制作为回报。结论胃黏膜下层可能是大鼠胰岛移植的理想部位。胃黏膜下层可能会缓解由 IL-1β 和 TNF-α 引发的早期胰岛功能障碍,这需要较少的移植胰岛数量并具有良好的血糖控制作为回报。
更新日期:2020-04-14
down
wechat
bug