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Wireless bioreactor for anaerobic cultivation of bacteria.
Biotechnology Progress ( IF 2.5 ) Pub Date : 2020-04-23 , DOI: 10.1002/btpr.3009
Ding-Shun Lin,Chih-Hsien Lee,Ya-Tang Yang

Anaerobic cultivation methods of bacteria are indispensable in microbiology. One methodology is to cultivate the microbes in anaerobic enclosure with oxygen‐adosrbing chemicals. Here, we report an electronic extension of such strategy for facultative anaerobic bacteria. The technique is based a bioreactor with entire operation including turbidity measurement, fluidic mixing, and gas delivery in an anaerobic enclosure. Wireless data transmission is employed and the anaerobic condition is achieved with gas pack. Although the technique is not meant to completely replace the anaerobic chamber for strict anaerobic bacteria, it provides a convenient way to bypass the cumbersome operation in anaerobic chamber for facultative anaerobic bacteria. Such a cultivation strategy is demonstrated with Escherichia coli with different carbon sources and hydrogen as energy source.

中文翻译:

用于细菌厌氧培养的无线生物反应器。

细菌的厌氧培养方法在微生物学中是必不可少的。一种方法是在厌氧环境中用吸氧化学品培养微生物。在这里,我们报告了这种兼性厌氧细菌策略的电子扩展。该技术基于生物反应器,其整个操作包括浊度测量、流体混合和厌氧外壳中的气体输送。采用无线数据传输,并通过气包实现厌氧条件。虽然该技术并不意味着完全取代严格厌氧菌的厌氧室,但它提供了一种方便的方法来绕过兼性厌氧菌在厌氧室中的繁琐操作。大肠杆菌证明了这种培养策略 以不同的碳源和氢为能源。
更新日期:2020-04-23
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