In 1 and 24 h after combined administration of TLR4 (LPS) and TLR3 (Poly I:C) ligands to CBA mice, the content of MSC in bone marrow increased to intermediate value between the levels attained by their individual injections. The content of osteogenic MSC assessed in 24 h postinjection corresponded to the control level in Poly I:C group, decreased in LPS group by 2.5 times relatively to the control, and increased by 1.6 times (relatively to control) after combined administration of the ligands. In 3 h after combined addition of LPS and Poly I:C in vitro to 12-day-old primary culture of mouse bone marrow stromal cells, the concentration of TNFα in culture medium was intermediate between the levels attained by their individual application. The data revealed dependence of activation of stromal tissue on intensity of innate immunity reactions; they also attested to marked elevation of osteogenicity of MSC pool after costimulation with Poly I:C and LPS, which can underlie augmented calcification of the tissues during combined viral and bacterial infections.

中文翻译：

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将 TLR4 (LPS) 和 TLR3 (Poly I:C) 配体联合给药至 CBA 小鼠可将成骨 MSC 的含量提高 1.6 倍，并将骨髓 MSC 的含量增加至其单独给药所获得的值之间的中间水平

在向 CBA 小鼠联合施用 TLR4 (LPS) 和 TLR3 (Poly I:C) 配体后 1 和 24 小时内，骨髓中的 MSC 含量增加到它们单独注射所达到的水平之间的中间值。注射后24小时成骨MSC的含量与Poly I:C组的对照水平相对应，LPS组相对于对照降低2.5倍，联合配体后增加1.6倍（相对于对照） . 在体外将 LPS 和 Poly I:C 联合添加到 12 天大的小鼠骨髓基质细胞原代培养物后 3 小时内，培养基中 TNFα 的浓度介于它们单独应用所达到的水平之间。数据揭示了基质组织的激活依赖于先天免疫反应的强度；