Considering the economic importance of the probiotics, industrial production of their biomass became important. Cane molasses, as an industrial byproduct, was used in this study to design a medium for biomass overproduction of a functionally probiotic strain, designated as Lactobacillus plantarum strain RPR42. The results showed that strain RPR42 can be best grown anaerobically in 22.5% cane molasses solution. Also, the findings of the single variable at a time experiments and either factorial design indicated that the optimal growth of strain RPR42 can be observed when beef extract, casein hydrolysate, and yeast extract were added into the medium. The central composite design experiments suggested a medium which was designated as cane molasses medium (CMM). Eventually, this medium contained 21.9% cane molasses, 30.72 g/L of a combined mixture of nitrogenous compounds: 0.0754% of a 1:1:1 mixture of polysorbates 20, 60, and 80, and 18.53 gr/L of the combined minerals. Such an optimized cane molasses-based medium supported a significant biomass production since a considerably high cell density, 13.8 g/L/24 h of dry biomass, of the strain was produced. Hence, cane molasses can be regarded as a promising substrate for industrial production purposes.

中文翻译：

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使用各种方法设计实验，在基于甘蔗糖蜜的培养基中高细胞密度生产功能性益生菌植物乳杆菌菌株 RPR42

考虑到益生菌的经济重要性，其生物质的工业生产变得重要。甘蔗糖蜜作为一种工业副产品，在本研究中被用于设计一种培养基，用于生产功能性益生菌菌株的生物量过剩，称为植物乳杆菌菌株 RPR42。结果表明菌株 RPR42 在 22.5% 甘蔗糖蜜溶液中厌氧生长效果最好。此外，单变量一次实验和任一因子设计的结果表明，当将牛肉提取物、酪蛋白水解物和酵母提取物添加到培养基中时，可以观察到菌株 RPR42 的最佳生长。中心复合设计实验提出了一种称为甘蔗糖蜜培养基 (CMM) 的培养基。最终，这种培养基含有 21.9% 的甘蔗糖蜜，30。72 g/L 含氮化合物的组合混合物：0.0754% 的 1:1:1 聚山梨醇酯 20、60 和 80 混合物，以及 18.53 gr/L 的组合矿物质。这种优化的基于甘蔗糖蜜的培养基支持显着的生物质生产，因为该菌株产生了相当高的细胞密度，13.8 g/L/24 h 的干生物质。因此，甘蔗糖蜜可被视为用于工业生产目的的有前途的基质。