Studies suggest that redox imbalance may be closely associated with pathological aging, contributing effectively to the genesis of several chronic diseases. One of the major defence enzymes against oxidation is Manganese-dependent superoxide dismutase (MnSOD) that acts within the mitochondria. The gene encoding this enzyme is polymorphic and Val16Ala variant is one of its most investigated polymorphisms regarding aging and oxidative stress. This study aimed to verify the occurrence of the MnSOD Val16Ala gene polymorphism association with markers of REDOX metabolism in the elderly of primary health care. A cross-sectional study was performed. The sample consisted of 270 elderly individuals from Family Health Strategy in the city of Porto Alegre, Rio Grande do Sul, Brazil (EMISUS). The following variables were investigated in all subjects: sociodemographic: gender, age, marital status, schooling and income; Anthropometric: weight, height, body mass index (BMI); REDOX markers: advanced oxidation protein products (AOPP), ischemia-modified albumin (IMA), nitric oxide metabolites (NOx), ferric reducing ability of plasma (FRAP) and malondialdehyde (MDA), MnSOD Val16Ala gene polymorphism. Val16Ala gene polymorphism was evaluated by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). Statistically significant associations were observed in the elderly with AA genotype compared to those with VV genotype, concerning AOPP (p = 0.023) and FRAP (p = 0.027) quartile frequencies, respectively. No statistically significant differences were observed between MnSOD genotypes with MDA, NOx and IMA oxidative markers. Val16Ala gene polymorphism is associated with AOPP and FRAP quartiles frequencies in the elderly of primary health care.

研究表明，氧化还原失衡可能与病理衰老密切相关，有效促成几种慢性疾病的发生。抗氧化的主要防御酶之一是线粒体内的锰依赖性超氧化物歧化酶（MnSOD）。编码该酶的基因具有多态性，Val16Ala变体是其针对衰老和氧化应激研究最多的多态性之一。本研究旨在验证在初级保健老年人中MnSOD Val16Ala基因多态性与REDOX代谢标志物的关联。进行了横断面研究。样本包括来自巴西南里奥格兰德州阿雷格里港市（EMISUS）的270名来自家庭健康策略的老年人。在所有主题中调查了以下变量：社会人口统计学：性别，年龄，婚姻状况，学历和收入；人体测量：体重，身高，体重指数（BMI）；REDOX标记：高级氧化蛋白产物（AOPP），局部缺血修饰的白蛋白（IMA），一氧化氮代谢产物（NOx），血浆铁还原能力（FRAP）和丙二醛（MDA），MnSOD Val16Ala基因多态性。通过聚合酶链反应和限制性片段长度多态性（PCR-RFLP）评估Val16Ala基因多态性。与AO基因型的老年人相比，AA基因型的老年人在统计学上具有显着相关性，分别涉及AOPP（p  = 0.023）和FRAP（p  = 0.027）四分位数频率。带有MDA，NO x和IMA氧化标记的MnSOD基因型之间没有观察到统计学上的显着差异。Val16Ala基因多态性与初级保健老年人中的AOPP和FRAP四分位数频率有关。