Peste des petits ruminants (PPR) is an acute, highly infectious, and highly pathogenic disease, which mainly damages small ruminants such as goats and sheep. Hemagglutinin protein (H), the main antigenic protein of peste des petits ruminants virus (PPRV), has been a hot spot in the research of genetic engineering vaccine for PPRV. In this study, the silkworm baculovirus surface display technology is combined with the transmembrane structure of the silkworm baculovirus envelope protein GP64 and different characteristics of the promoters to display four kinds of fusion proteins, which contain Pph-H, Pph-HJ, Pie1-H, and Pie1-HJ. The fusion proteins displayed on baculovirus surface have been detected by western blotting, cell surface immunofluorescence, and immunogold electron microscopy. In addition, the dominant form of PPR H displayed on baculovirus surface has been determined which is fusion protein mediated by Pph containing the hemagglutinin protein and full-length GP64, Pph-H. Furthermore, by comparing the fluorescence intensity of binding of hemagglutinin protein and signaling lymphocyte activation molecules (SLAM) in Vero-SLAM cells by immunocytochemistry, Pph-H can be combined with the receptor protein of PPRV, SLAM. It provides technical support for displaying the different structure of hemagglutinin and exploring the key sites of hemagglutinin and SLAM binding. Meanwhile, it is important for exploring the pathogenesis and immune mechanism of PPRV.

中文翻译：

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与小反刍动物病毒血凝素受体 SLAM 和杆状病毒表面显示的相互作用。

小反刍兽疫（PPR）是一种急性、高传染性、高致病性的疾病，主要危害山羊、绵羊等小型反刍动物。血凝素蛋白(H)是小反刍兽疫病毒(PPRV)的主要抗原蛋白，一直是PPRV基因工程疫苗研究的热点。本研究利用家蚕杆状病毒表面展示技术，结合家蚕杆状病毒包膜蛋白GP64的跨膜结构和启动子的不同特性，展示了四种融合蛋白，分别包含P ph -H、P ph -HJ、P ie1 -H 和 P ie1-HJ。已通过蛋白质印迹、细胞表面免疫荧光和免疫金电子显微镜检测到杆状病毒表面显示的融合蛋白。此外，已经确定了杆状病毒表面上展示的 PPR H 的主要形式，它是由含有血凝素蛋白的 P ph和全长 GP64、P ph -H介导的融合蛋白。此外，通过免疫细胞化学比较 Vero-SLAM 细胞中血凝素蛋白与信号淋巴细胞活化分子 (SLAM) 结合的荧光强度，P ph-H可以与PPRV、SLAM的受体蛋白结合。为展示血凝素的不同结构，探索血凝素与SLAM结合的关键位点提供技术支持。同时，对于探索PPRV的发病机制和免疫机制具有重要意义。