Monoterpenes are important components of plant essential oils and have long been used as raw materials for spices and food flavorings. A number of studies have been performed to increase the content of monoterpenes in plants, but no obvious effect was observed. Exchange was observed between the methylerythritol phosphate (MEP) and mevalonic acid (MVA) metabolic pathways, which produce monoterpenes and sesquiterpenes, respectively. However, the specific details of the communication have not been elucidated. In the present study, we investigated the effects of overexpressing Litsea cubeba (Lour.) Persoon 3-hydroxy-3-methylglutaryl-coenzyme A synthase (LcHMGS) on the production of monoterpenes and sesquiterpenes. In addition, we also explored the flow of metabolic flux between the MEP and MVA pathways. We cloned LcHMGS and analyzed its expression pattern in various tissues. The overexpression of LcHMGS significantly increased the species and content of monoterpenes and sesquiterpenes. In addition, LcHMGS overexpression in plants induced such phenotypes as excessive growth, enlarged vegetative organs and early flowering by elevating the GA₃ content. Our results demonstrate a metabolic engineering strategy to improve the yield of monoterpenes and sesquiterpenes and simultaneously increase the biomass of plants.

中文翻译：

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3-羟基-3-甲基戊二酰辅酶A合酶基因LcHMGS的过表达有效地增加了单萜和倍半萜的产量。

单萜是植物精油的重要成分，长期以来一直用作香料和食品调味料的原料。已经进行了许多研究来增加植物中单萜的含量，但是没有观察到明显的效果。观察到甲基赤藓醇磷酸酯（MEP）和甲羟戊酸（MVA）代谢途径之间的交换，它们分别产生单萜和倍半萜。但是，通讯的具体细节尚未阐明。在本研究中，我们调查了过表达Litsea cubeba（Lour。）季风3-羟基-3-甲基戊二酰辅酶A合酶（LcHMGS）的影响）生产单萜和倍半萜。此外，我们还探讨了MEP和MVA途径之间的代谢通量流。我们克隆了LcHMGS，并分析了其在各种组织中的表达模式。LcHMGS的过表达显着增加了单萜和倍半萜的种类和含量。此外，LcHMGS在植物中的过表达通过增加GA ₃含量诱导表型如过度生长，营养器官增大和早期开花。我们的结果证明了代谢工程策略可以提高单萜和倍半萜的产量，同时增加植物的生物量。