Previous evaluations of the effect of ultraviolet (UV) light on Cryptosporidium parvum oocysts have been limited to a single strain—the Iowa strain. This study investigated the response of five strains of C. parvum to UV. A collimated beam apparatus was used to apply controlled doses of monochromatic (254 nm) UV to oocysts of the Iowa, Moredun, Texas A&M, Maine, and Glasgow strains. Irradiation was measured using a calibrated radiometer and sensor. Inactivation was quantified through animal infectivity by inoculation of cohorts of CD‐1 neonatal mice with UV‐treated and untreated control oocysts of each strain followed by examination of intestinal sections for infection using hemotoxylin and eosin staining. A UV light dose of 10 mJ/cm2 achieved at least 4‐log10 inactivation of all strains evaluated. All five strains of C. parvum were shown to be highly susceptible to low levels of UV light.

中文翻译：

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五株小隐孢子虫卵囊对紫外线的敏感性

先前对紫外线 (UV) 光影响的评估小隐孢子虫卵囊仅限于单一菌株——爱荷华菌株。本研究调查了五种菌株的反应微小念珠菌到紫外线。使用准直光束装置将受控剂量的单色（254 nm）紫外线应用于爱荷华州、莫雷登、德克萨斯农工大学、缅因州和格拉斯哥菌株的卵囊。使用校准的辐射计和传感器测量辐射。通过用经紫外线处理和未经处理的每种菌株的对照卵囊接种 CD-1 新生小鼠组，然后使用苏木精和伊红染色检查肠道切片的感染情况，通过动物感染性对灭活进行量化。10 mJ/cm2 的紫外线剂量2达到至少 4-log10评估所有菌株的失活。所有五株微小念珠菌研究表明，它们对低水平的紫外线高度敏感。