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The use of PCR and BsmAI restriction combination targeting wciP gene to determine serotype 6A, 6B, 6C and 6D Streptococcus pneumoniae.
Journal of Microbiological Methods ( IF 1.7 ) Pub Date : 2020-04-01 , DOI: 10.1016/j.mimet.2020.105909 Wisnu Tafroji 1 , Felicia Monica Bernadette 2 , Ernawati A Giri Rachman 2 , Dodi Safari 1
Journal of Microbiological Methods ( IF 1.7 ) Pub Date : 2020-04-01 , DOI: 10.1016/j.mimet.2020.105909 Wisnu Tafroji 1 , Felicia Monica Bernadette 2 , Ernawati A Giri Rachman 2 , Dodi Safari 1
Affiliation
We designed a sensitive and reliable method to distinguish serogroup 6 using PCR followed by enzymatic restriction digest. We discovered that this serotyping method was able to distinguish serotypes 6A, 6B, 6C, and 6D based on the recognition site of BsmAI in the wciP region.
中文翻译：
使用靶向wciP基因的PCR和BsmAI限制性酶切组合测定肺炎链球菌的血清型6A，6B，6C和6D。
我们设计了一种敏感而可靠的方法，使用PCR和酶切限制酶切消化来区分血清群6。我们发现，这种血清分型方法能够基于wciP区域中BsmAI的识别位点区分血清型6A，6B，6C和6D。
更新日期：2020-04-01
中文翻译：
使用靶向wciP基因的PCR和BsmAI限制性酶切组合测定肺炎链球菌的血清型6A，6B，6C和6D。
我们设计了一种敏感而可靠的方法，使用PCR和酶切限制酶切消化来区分血清群6。我们发现，这种血清分型方法能够基于wciP区域中BsmAI的识别位点区分血清型6A，6B，6C和6D。
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