Wastewater (WW) carry considerable amount of chemicals that could have mutagenic or cytotoxic effect from hospital discharges to aquatic environment. Our objective was to determinate the possible mutagenic and toxic effects of hospital originated WWs and effectiveness of the wastewater treatment plants (WTP) functions. In the study the mutagenic and cytotoxic potential of three hospitals and influent/effluent of a treatment plant WW collected in Istanbul and was examined using AMES, XTT, and lactate dehydrogenase (LDH) assays. Mutagenic effects were detected at both hospital discharges and advanced biological wastewater plant. We observed no cytotoxic effect in fibroblasts for LDH and XTT assays whereas high cytotoxicity for all samples was found in hepatocytes by XTT assay. According to the results even if advanced technology is used for treatment of WW, mutagenic and cytotoxic effects still remain, and the present technologies need to be further improved.

中文翻译：

---

伊斯坦布尔废水的细胞毒性和致突变性评估：医院和高级废水处理厂的数据。

废水（WW）携带大量化学物质，这些化学物质从医院排放到水生环境可能具有诱变或细胞毒性作用。我们的目标是确定医院起源的WW可能引起的诱变和毒性作用以及废水处理厂（WTP）功能的有效性。在这项研究中，在伊斯坦布尔收集了三家医院以及污水处理厂WW的进/出水的致突变性和细胞毒性潜力，并使用AMES，XTT和乳酸脱氢酶（LDH）分析进行了检查。在医院出院和先进的生物废水处理厂均检测到致突变作用。我们观察到对于LDH和XTT分析，在成纤维细胞中没有细胞毒性作用，而通过XTT分析，在肝细胞中发现所有样品的细胞毒性都很高。