Osteoblast differentiation is a key process in bone homeostasis. Mutations in plastin 3 have been reported to be responsible for X-linked osteoporosis. Plastin 3 and plastin 2 act synergistically to regulate osteoblast differentiation. However, the bone-related function of plastin 1, another family member of plastins, has not been assessed. In this study, we addressed the functional importance of plastin 1 in osteoblasts. We characterized the expression patterns of plastin 1 during osteoblast differentiation and revealed its important role in this process. In both HEK 293T and hFOB1.19 cells, plastin 1 was demonstrated to regulate intracellular Ca²⁺. Accordingly, we revealed that higher Ca²⁺ concentration promotes osteoblast differentiation. Finally, we found that plastin 1 may play a compensatory role in osteoporosis patients with plastin 3 deficiency. Together, our results indicate that plastin 1 promotes osteoblast differentiation by regulating intracellular Ca²⁺. Our work sheds new light on the role played by plastins in bone homeostasis.

中文翻译：

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Plastin 1通过调节细胞内Ca2促进成骨细胞分化。

成骨细胞分化是骨稳态的关键过程。已有报道，质体3的突变是造成X连锁骨质疏松的原因。Plastin 3和plastin 2协同作用来调节成骨细胞的分化。但是，尚未评估另一种塑性素家族成员–塑性素1的骨相关功能。在这项研究中，我们解决了成骨细胞中plastin 1的功能重要性。我们表征了成骨细胞分化过程中增塑素1的表达模式，并揭示了其在此过程中的重要作用。在HEK 293T和hFOB1.19细胞中，均显示增塑素1调节细胞内Ca ²⁺。因此，我们发现较高的Ca ²⁺浓度促进成骨细胞分化。最后，我们发现增塑酶1在缺乏增塑剂3的骨质疏松症患者中可能起补偿作用。在一起，我们的结果表明，增塑素1通过调节细胞内Ca ²⁺促进成骨细胞分化。我们的工作为增塑素在骨稳态中的作用提供了新的思路。