当前位置：
X-MOL 学术
›
AIDS Res. Hum. Retrovir.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Multiple HIV-1 Genotypes Circulating Among College Students in Nanjing, China.
AIDS Research and Human Retroviruses ( IF 1.5 ) Pub Date : 2020-07-02 , DOI: 10.1089/aid.2019.0288 Wei Li 1 , Zhengping Zhu 2 , JinJin Chu 1 , You Ge 1 , Yuanyuan Xu 2 , Sushu Wu 2 , Yan He 1 , Qian Ni 1 , Yangyang Liu 1 , Rui Li 1 , Xiaoshan Li 3 , Pingmin Wei 1
AIDS Research and Human Retroviruses ( IF 1.5 ) Pub Date : 2020-07-02 , DOI: 10.1089/aid.2019.0288 Wei Li 1 , Zhengping Zhu 2 , JinJin Chu 1 , You Ge 1 , Yuanyuan Xu 2 , Sushu Wu 2 , Yan He 1 , Qian Ni 1 , Yangyang Liu 1 , Rui Li 1 , Xiaoshan Li 3 , Pingmin Wei 1
Affiliation
College students are disproportionately affected by HIV-1 in China. However, little is known about the genetic characteristics of HIV-1 among this population. A molecular epidemiological investigation was conducted among the newly diagnosed antiretroviral therapy-naïve HIV-1-infected individuals during 2015–2019 in Nanjing city, China. The pol fragment (HXB2: 2,253–3,311) was obtained by HIV-1 RNA extraction and gene amplification, and subjected to genotyping, recombination analysis, and phylogenetic inference. A total of 945 pol sequences from 226 students and 719 nonstudents were successfully amplified. Multiple genotypes were identified in students, including CRF01_AE (37.66%), CRF07_BC (32.90%), CRF55_01B (5.63%), CRF68_01B (3.46%), CRF67_01B (3.03%), subtype B (1.73%), and CRF58_01B (1.30%) and unique recombinant forms (URFs) of 01C_like (7.08%), 0107_like (3.98%), 01BC_like (2.21%), and 01B_like (1.33%). The distribution of genotypes among students was similar to that among nonstudents. The estimated mean evolutionary rate of URFs was 2.89 × 10−3 [95% Bayesian credible interval: 1.89–3.90] nucleotide substitutions/site/year. Approximately 64% (21/33) of URFs among students were located in three major clusters (0107_like, 01C_like 1, and 01C_like 2 clusters), which had recent time to the most recent common ancestors and low mean genetic distance, and presumably originated from Nanjing (posterior probability ≥0.99, state probability ≥0.9). Among 226 students with pol segments, the prevalence of primary and transmitted drug resistance mutations was 15.93% and 3.98%, respectively. The rapid evolution of multiple HIV-1 genotypes and high prevalence of URFs circulating among students in Nanjing emphasized the necessity of comprehensive surveillance for HIV-1 transmission among this population.
更新日期：2020-07-03
京公网安备 11010802027423号