Abstract

Eriocalyxin B, an ent-Kaurene diterpenoid extracted from a traditional Chinese herb Isodon eriocalyx, has been shown to possess multifunctional activities such as anti-cancer and anti-inflammatory. However, the function and mechanism of the compound in adipocyte differentiation is still unknown. Here we reported that eriocalyxin B blunted adipogenesis remarkably by inhibiting the accumulation of lipid droplets, triglycerides and the expressions of adipogenesis-related factors, including C/EBPβ, C/EBPα, PPARγ, and FABP4. Moreover, we showed that the inhibition might be the consequence of cell cycle being arrested at the G2/M phase during the mitotic clonal expansion of adipocyte differentiation, most likely by suppressing mRNAs and proteins of CDK1, CDK2, Cyclin A and Cyclin B1. Overall, we conclude that eriocalyxin B is capable of inhibiting adipocyte differentiation at the early stage through downregulating the proteins involved in cell cycle progression.

Graphic Abstract

中文翻译：

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Eriocalyxin B 通过细胞周期阻滞抑制 3T3-L1 脂肪细胞中的脂肪生成。

 抽象的

Eriocalyxin B 是从传统中药Isodon eriocalyx中提取的一种对映贝壳杉烯二萜类化合物，已被证明具有抗癌和抗炎等多功能活性。然而，该化合物在脂肪细胞分化中的功能和机制仍不清楚。在这里，我们报道了 eriocalyxin B 通过抑制脂滴、甘油三酯的积累和脂肪生成相关因子（包括 C/EBPβ、C/EBPα、PPARγ 和 FABP4）的表达来显着减弱脂肪生成。此外，我们发现这种抑制可能是脂肪细胞分化的有丝分裂克隆扩张过程中细胞周期被阻滞在 G2/M 期的结果，很可能是通过抑制 CDK1、CDK2、细胞周期蛋白 A 和细胞周期蛋白 B1 的 mRNA 和蛋白来实现的。总的来说，我们得出结论，eriocalyxin B 能够通过下调参与细胞周期进程的蛋白质来抑制早期脂肪细胞分化。

 图文摘要