The aim of this study was to compare the microbial community structure and diversity in powdery mildew-infected and noninfected strawberry plant rhizosphere soils in the greenhouse based on variations in the 16S rRNA gene V3–V4 and fungal ITS2 regions by Illumina amplicon sequencing. Powdery mildew infection reduced the number of operational taxonomic units (OTUs) and prokaryotic and fungal community richness/diversity indexes in the rhizosphere soils compared with those in healthy plant soils. Furthermore, 3543 prokaryotic and 581 fungal OTUs were obtained at the 97% similarity level. Proteobacteria, Actinobacteria, Bacteroidetes, Acidobacteria, and Chloroflexi were the dominant bacterial phyla; Woesearchaeota_DHVEG-6, Bathyarchaeota, and Thaumarchaeota were the dominant archaea; and Ascomycota, Basidiomycota, unclassified_fungi, and Zygomycota were the dominant fungal phyla. Their proportions differed significantly among samples. Wolbachia , Devosia , Pseudolabrys , Streptomyces , and Rhizomicrobium were the most abundant bacterial genera; their proportions differed significantly among samples. Most Pseudomonas , Streptomyces , and ‘norank’ group members might be potential antagonistic microorganisms of powdery mildew pathogens, and Wolbachia and Rickettsia might be pathogen-transmitting vectors. Microascus , Clitopilus , and Ciliophora were the dominant fungi, and their community structures and abundances significantly differed among samples. Microascus , Talaromyces , Zopfiella , and Cryptococcus were relatively more abundant in the powdery mildew-infected strawberry plant rhizosphere soils. Fusarium , Trichoderma , Clitopilus , and ‘unclassified’ group members may be potential antagonistic populations. The results suggested that powdery mildew-infected strawberry fruits and plants cannot be consumed. This report is the first study to illustrate differences in the rhizosphere soil prokaryotic and fungal communities between powdery mildew-infected and noninfected strawberry plants in a greenhouse.

中文翻译：

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高通量测序技术比较温室中白粉病感染和未感染草莓植株根际土壤微生物群落结构和多样性

本研究的目的是通过 Illumina 扩增子测序，基于 16S rRNA 基因 V3-V4 和真菌 ITS2 区域的变化，比较温室中感染白粉病和未感染草莓植物根际土壤的微生物群落结构和多样性。与健康植物土壤相比，白粉病感染减少了根际土壤中操作分类单元 (OTU) 和原核和真菌群落丰富度/多样性指数的数量。此外，以 97% 的相似度获得了 3543 个原核和 581 个真菌 OTU。Proteobacteria、Actinobacteria、Bacteroidetes、Acidobacteria 和 Chloroflexi 是优势菌门；Woesearchaeota_DHVEG-6、Bathyarchaeota 和 Thaumarchaeota 是优势古菌；和子囊菌门、担子菌门、unclassified_fungi、和接合菌门是优势真菌门。它们的比例在样本之间存在显着差异。Wolbachia、Devosia、Pseudolabrys、Streptomyces和Rhizomicrobium是最丰富的细菌属；它们的比例在样本之间存在显着差异。大多数假单胞菌、链霉菌和'norank'组成员可能是白粉病病原体的潜在拮抗微生物，沃尔巴克氏体和立克次氏体可能是病原体传播的载体。Microascus、Clitopilus 和 Ciliophora 是优势真菌，它们的群落结构和丰度在样品之间存在显着差异。微子囊菌、Talaromyces、Zopfiella 和 Cryptococcus 在白粉病感染的草莓植物根际土壤中相对较多。镰刀菌 , 木霉 , Clitopilus , 和“未分类”的群体成员可能是潜在的敌对群体。结果表明，不能食用受白粉病感染的草莓果实和植物。本报告是第一个说明温室中感染白粉病和未感染草莓植物的根际土壤原核和真菌群落差异的研究。