Beaded nanofiber is a promising fibrous structure could act as drug delivery system with sustained drug release for regulating cell behaviors used in tissue engineering. Poly (L-lactic acid-co-ε-caprolactone) (PLCL) beaded nanofiber with core-shell structure (130 ± 30 nm) was fabricated and bovine serum albumin (BSA) was encapsulated into the inner layer. The surface morphology and characteristic were evaluated by scanning electron microscopy (SEM), inverted fluorescence microscopy and water contact angle test. Degradation analyses suggested that PLCL/BSA core-shell @ beaded nanofibers could maintain the fibrous framework during 3 weeks. The biocompatibility was investigated by in vitro cultivation of human mesenchymal stem cells (hMSCs) on the surface of PLCL/BSA core-shell @ beaded nanofibers. The proliferation of hMSCs was tested using alamar blue reagent and the spreading morphology of cells was observed by SEM. Corresponding results suggested that beaded nanofibers with core-shell structure could effectively support the attachment and proliferation of cells. PLCL beaded nanofiber with core-shell structure would work as a promising candidate for drug release system and tissue engineering.

中文翻译：

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具有核壳结构的 BSA 负载珠串纳米纤维支架在组织工程中的应用。

珠状纳米纤维是一种很有前途的纤维结构，可以作为药物递送系统，持续释放药物，用于调节组织工程中使用的细胞行为。制造了具有核壳结构（130±30 nm）的聚（L-乳酸-共-ε-己内酯）（PLCL）珠状纳米纤维，并将牛血清白蛋白（BSA）封装在内层中。通过扫描电子显微镜(SEM)、倒置荧光显微镜和水接触角测试评估表面形貌和特性。降解分析表明，PLCL/BSA 核壳@珠状纳米纤维可以在 3 周内保持纤维框架。通过在 PLCL/BSA 核壳@珠状纳米纤维表面体外培养人间充质干细胞 (hMSCs) 来研究生物相容性。用阿拉玛蓝试剂检测hMSCs的增殖，扫描电镜观察细胞的铺展形态。相应的结果表明，具有核壳结构的珠状纳米纤维可以有效地支持细胞的附着和增殖。具有核壳结构的 PLCL 珠状纳米纤维将作为药物释放系统和组织工程的有希望的候选者。