The objective of this study is to investigate the utility of gelatin hydrogel-fragmented fibers (GHFF) as a material to suppress the shrinkage of cell sheets, which often happens upon detaching from a culture plate. The GHFF were fabricated by cutting gelatin hydrogel nonwoven fabrics. MC3T3-E1 cells were simply mixed with different amounts of GHFF, followed by culturing to formulate the cell sheet homogeneously incorporating GHFF. When detached from the culture plate, the cell sheet formulated without GHFF shrunk while the area became about 23% of the original one before detachment. On the contrary, the cell sheet formulated with GHFF hardly shrunk. The lactate/glucose ratio of a metabolic activity was significantly lower and the adenosine triphosphate (ATP) production was higher for the cell sheet formulated with the GHFF than that obtained without the GHFF. An osteogenic activity was high for the cell sheet formulated with the GHFF compared with that obtained without the GHFF. The GHFF addition was a simple and promising method to fabricate active cell sheets without size change. Impact Statement This study introduces the utility of gelatin hydrogel-fragmented fibers (GHFF) for cell sheet engineering. Upon detaching from the culture plate, the cell sheet formulated without GHFF shrunk, while the area became about 23% of the original one before detachment. On the contrary, the cell sheet formulated with GHFF hardly shrunk. The GHFF allowed cell sheets to enhance the metabolic and osteogenic activities. The GHFF addition was a simple and promising method to fabricate active cell sheets without size change.

中文翻译：

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明胶水凝胶破碎的纤维抑制细胞片的收缩。

这项研究的目的是研究明胶水凝胶碎片纤维（GHFF）作为抑制细胞片收缩的材料的实用性，这种收缩通常发生在与培养板分离时。GHFF是通过切割明胶水凝胶非织造布制成的。将MC3T3-E1细胞简单地与不同量的GHFF混合，然后进行培养以配制均匀掺入GHFF的细胞片。当从培养板上取下时，没有GHFF的细胞片会收缩，而面积却变成了原先的23％。相反，用GHFF配制的细胞片几乎不收缩。与没有GHFF的细胞片相比，使用GHFF的细胞片的代谢活性的乳酸/葡萄糖比率明显较低，三磷酸腺苷（ATP）的产生较高。与不使用GHFF的细胞片相比，使用GHFF的细胞片的成骨活性高。GHFF的添加是一种简单且有前途的方法，可用于制造尺寸不变的活性细胞片。影响陈述这项研​​究介绍了明胶水凝胶片段化纤维（GHFF）在细胞片工程中的用途。从培养板上取下后，没有GHFF配制的细胞片会收缩，而面积却变成了分离前原始细胞片的23％。相反，用GHFF配制的细胞片几乎不收缩。GHFF使细胞片增强了代谢和成骨活性。GHFF的添加是一种简单且有前途的方法，可用于制造尺寸不变的活性细胞片。